荧光团杂化纳米SiO_2微球作为生物标记探针的应用研究  

Nanometer Fluorescent Hybrid Silica Particle as Ultrasensitive and Photostable Biological Labels

作　　者：曲会英[1] 杨黄浩[1] 林鹏[1] 李顺华[1] 杨薇[1] 许金钩[1] 

机构地区：[1]厦门大学化学化工学院现代分析教育部重点实验室,厦门大学化学系厦门361005

出　　处：《高等学校化学学报》2003年第3期422-424,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 175 0 2 1)资助

摘　　要：Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. In this paper, we develop a kind of novel nanometer-sized fluorescent hybrid silica(NFHS) particles used as a sensitive and photostable fluorescent probe in biological staining and diagnostics. The NFHS particles are prepared by controlled hydrolysis of the fluorophore silica precursor using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. In comparison with single organic fluorophores without incorporation, these nanoparticle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission(blinking). The NFHS particles have also shown unique advantages over the existing common organic fluorophores, quantum dots, and latex-based fluorescent particles for biomolecule recognition in the following four major points: easy preparation, good photostability, high sensitivity, and low toxicity. The approach proposed in this article for making NFHS nanoparticles is a general one, and it is not restricted to a particular type of fluorophore molecule as selected in this study.Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. In this paper, we develop a kind of novel nanometer-sized fluorescent hybrid silica(NFHS) particles used as a sensitive and photostable fluorescent probe in biological staining and diagnostics. The NFHS particles are prepared by controlled hydrolysis of the fluorophore silica precursor using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. In comparison with single organic fluorophores without incorporation, these nanoparticle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission(blinking). The NFHS particles have also shown unique advantages over the existing common organic fluorophores, quantum dots, and latex-based fluorescent particles for biomolecule recognition in the following four major points: easy preparation, good photostability, high sensitivity, and low toxicity. The approach proposed in this article for making NFHS nanoparticles is a general one, and it is not restricted to a particular type of fluorophore molecule as selected in this study.

关 键 词：荧光团 杂化 纳米SiO2微球 生物标记探针 荧光免疫分析 

分 类 号：R446.6[医药卫生—诊断学] O657.3[医药卫生—临床医学]