电喷雾质谱动力学方法测定磷酰化丙丙二肽的气相质子亲和能  

Determination of the Gas-Phase Proton Affinity of N-Dipropyloxy-phosphoryl Alkyl-alanine by Electrospray Ionization Mass Spectrometry with the Kinetic Method

作　　者：陈晶[1] 陈益[1] 江洋[1] 赵玉芬[1] 

机构地区：[1]清华大学生命科学与工程研究院化学系

出　　处：《高等学校化学学报》2003年第3期459-461,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学重点基金 (批准号 :2 0 175 0 2 6)资助

摘　　要：In this work, the change of gas-phase proton affinity of alkyl-alanine through the phosphorylation reaction was studied by the electrospray ionization mass spectrometry with kinetic method. It was proved that the phosphorylation could increase the proton affinity of small peptide. A proposal that proton affinity increase might be responsible for the sensitivity improvement of small peptide in mass spectrometry has been provided.In this work, the change of gas-phase proton affinity of alkyl-alanine through the phosphorylation reaction was studied by the electrospray ionization mass spectrometry with kinetic method. It was proved that the phosphorylation could increase the proton affinity of small peptide. A proposal that proton affinity increase might be responsible for the sensitivity improvement of small peptide in mass spectrometry has been provided.

关 键 词：电喷雾质谱 动力学方法 测定 磷酰化 丙丙二肽 气相质子亲和能 

分 类 号：Q516[生物学—生物化学] Q503