高效液相色谱法测定芪麝颈康丸中青藤碱的含量  

Determination of Sinomenine in Qishejingkang Pills by HPLC

作　　者：刘梅[1] 张宁[2] 王拥军[1] 施杞[1] 沈培芝[1] 徐宇[1] 

机构地区：[1]上海中医药大学龙华医院骨伤科研究所,200032 [2]上海中医药大学,200032

出　　处：《时珍国医国药》2003年第2期67-68,共2页Lishizhen Medicine and Materia Medica Research

基　　金：上海市科委新药项目 (No .98431 91 0 1 )

摘　　要：目的 :建立测定芪麝颈康丸中青藤碱含量的方法。方法 :采用HPLC法。样品用 70 %乙醇超声提取 ,在C18柱上以甲醇 乙腈 水 二乙胺 ( 18∶14∶5 8∶0 .1)为流动相 ,在波长 2 65nm处检测。结果 :其线性范围为 0 .378～ 1.89μg ,回归方程为Y =175 4.5 6X -10 .35 ,r =0 .9996,回收率为 99.44% ,RSD为 1.82 %。结论 :该方法简便 ,准确 ,重现性好 。Objective:To establish a method for the determination of the contents of Sinomenine in Qishejingkang bills.Methods:The sample was shaken in an ultrasonic bath by 70% Ethyl alcohol. The contents were determined by HPLC, with column C 18 as stationary phase, methanol-acetonitrile-water-Diethyl Amine(18∶14∶58∶0.1)as mobile phase and UV detector at 265 nm.Results:The content of Sinomenine had a good linearity at the range of 0.378～1.89 μg,Y=1 754.56X-10.35,r=0.999 6. The average recovery was 99.44% and RSD was 1.82%.Conclusion:The method is rapid, reliable and simple, and can be used in the quality control of Qishejingkang pills.

关 键 词：高效液相色谱法 芪麝颈康丸 青藤碱 含量 质量控制 

分 类 号：R284.2[医药卫生—中药学]