STUDIES ON THE ISOLATION PURIFICATION AND CHARACTERIZATION OF THE FUCOIDAN-GALACTOSAN SULFATE (FGS) FROM LAMINARIA JAPONICA  

作　　者：TANG Zhihong LI Fuchuan XU Zhongping WANG Hairen ZHANG Guozhi 

机构地区：[1]College of Life Science,Shandong University,Jinan 250100 [2]Yantai Kangtai Medicine Product Corp.,Shandong province 264006

出　　处：《Chinese Journal of Reactive Polymers》2000年第2期151-155,共5页中国反应性高分子（英文版）

摘　　要：FGS, isolated from the water solution of enzymolyzed laminaria japonica, is a mixture of acid heteroglycans. Four fractions F1, F2, F3, and F4 were obtained from FGS by ion exchange chromatography. After further purified by gel filtration chromatography on a sepharose 2B and 6B column, we obtained F9, F10, F11, and F12. They showed single band when identified by electrophoresis. The molecular weight of F9, F10, F11, and F12 was estimated to be 216, 120, 138 and 140KD respectively. They containedа-glucosidic bond by IR and 1H-NMR analysis. The typical absorption peaks of these polysaccharides were showed in UV and IR spectra. These polysaccharides contained rha, fuc, man, gal, and uronate when identified by paper chromatography (p.c) and gas chromatography (GC). The molar ratio of these sugars was also assayed.FGS, isolated from the water solution of enzymolyzed laminaria japonica, is a mixture of acid heteroglycans. Four fractions F1, F2, F3, and F4 were obtained from FGS by ion exchange chromatography. After further purified by gel filtration chromatography on a sepharose 2B and 6B column, we obtained F9, F10, F11, and F12. They showed single band when identified by electrophoresis. The molecular weight of F9, F10, F11, and F12 was estimated to be 216, 120, 138 and 140KD respectively. They containedа-glucosidic bond by IR and 1H-NMR analysis. The typical absorption peaks of these polysaccharides were showed in UV and IR spectra. These polysaccharides contained rha, fuc, man, gal, and uronate when identified by paper chromatography (p.c) and gas chromatography (GC). The molar ratio of these sugars was also assayed.

关 键 词：Laminaria japonica Fucoidan-Galactosan sulfate ISOLATION PURIFICATION Chromatograph( 

分 类 号：O635.1[理学—高分子化学] Q592[理学—化学]