17β-雌二醇对去卵巢SD大鼠离体骨组织微损伤的影响  

Effect of 17β-estradiol on Bone Microdamage in Vitro of Ovariectomized SD Rat

作　　者：戴如春[1] 廖二元[1] 杨川[1] 伍贤平[1] 苏欣[1] 邓小戈[1] 彭健[1] 

机构地区：[1]中南大学湘雅二院代谢内分泌研究所,湖南长沙410011

出　　处：《中国康复》2003年第1期6-9,共4页Chinese Journal of Rehabilitation

基　　金：国家"九五"攻关项目 ( 96 - 90 6- 0 5 - 0 5 );国家自然科学基金项目 ( 39970 347);国家"十五"攻关项目( 2 0 0 1BA70 2B0 3);湖南省社会发展科研计划 ( 99SSY10 0 9- 10 )

摘　　要：目的 :观察 17β 雌二醇对不同时期去卵巢SD大鼠离体骨组织微损伤的影响 ,评价抗骨质疏松药物的防治效果及评价指标。方法 :10月龄SD大鼠 90只 ,随机分为 3组各 30只 ,每组分成去卵巢组 (OVX组 )、17β 雌二醇替代组(EST组 )和对照组 (SHAM组 ) 3个小组 ,分别于去卵巢后 3、15和 2 1周时处死大鼠 ,右侧胫骨上端行常规骨形态计量学观察 ,对第 4离体椎骨进行疲劳损伤后观测微损伤。结果 :疲劳损伤后每块骨组织磨片均可见微损伤 ,以微破裂最常见 ,各组间及小组间比较差异均无显著性。骨小梁单位面积百分率微破裂长度和数目在 3周时出现OVX组明显低于EST组 ,与SHAM组比较差异无显著性 (P >0 0 5 ) ;15周时 3组差异消失 ,2 1周时OVX组明显大于其它 2组 (P <0 0 5 ) ,EST组与SHAM组比较差异均无显著性 (P >0 0 5 )。结论 :10月龄SD大鼠去卵巢后早期尚不表现出骨结构性能的下降 ,17β 雌二醇早期应用可使骨结构性能提高 ,且应用至去卵巢后较晚时期显示出良好的抗骨结构性能下降效果。单位骨小梁面积百分率的微破裂长度和数目可反映药物对骨质疏松的影响 ,可作为骨结构性能的评价指标。观察时间以去卵巢后 2 1周较佳。Objective:To observe the effect of 17β-estradiol (E 2) on bone microdamage in ovariectomized SD rats at different stages, evaluate the preventive and treating effectiveness of anti-osteoporostics, as well as to find the best parameters for bone biomechanic assessment in E 2-treated rats. Methods:Ninety SD 10-month-aged female rats were divided into 3 groups: group A, ovariectomized (OVX); group B, sham-operated (SHAM) and group C, E 2 (10 μg/kg every day). At the 3rd,15th and 21st week after ovariectomy the rats were killed respectively. The superior extremities of right tibia were used for bone morphometry and the 4th vertebrae for fatigue damage test by SL-2000 bone fatigue experiment device. The parameters of microdamage were measured. Results:Microdamage could be observed in each sample and microcrack was the most usual damaged type (48.64±8.41 μm). Significant difference of length and number of microcrack per percent of trabecular area was observed (P<0.05) between OVX group and E 2 group at the 3rd and 21st week, but not between OVX and SHAM groups at the 15th week. Conclusion:E 2 could improve bone biological quality at the earlier stage and prevent it from deterioration later. The length and number of microcrack per percent of trabecular area could be used as bone biomechanical marker to evaluate the effectiveness of anti-osteoporostics.

关 键 词：17Β-雌二醇 微损伤 卵巢切除术 SD大鼠 骨 

分 类 号：R965[医药卫生—药理学]