氟哌利多对大鼠脑缺血海马CA1区锥体细胞持续钠通道电流的影响  

Effects of droperidol on the enhancement of persistent sodium currents induced by in vitro ischemia-like condition in isolated rat CA1 paramidal neurons

作　　者：焦志华[1] 庄心良[1] 王士雷[1] 张一 颜涛[1] 

机构地区：[1]上海市第一人民医院麻醉科,上海200080

出　　处：《中国临床药理学与治疗学》2003年第1期48-51,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :研究氟哌利多对大鼠脑缺血海马CA1区锥体细胞持续钠通道电流的影响。方法 :酶消化法急性分离SD大鼠 (10～ 14d)海马CA1区锥体细胞 ,通过低氧和无糖法制备神经元缺血模型 ,全细胞膜片钳技术记录氟哌利多对脑缺血时锥体细胞持续性钠通道电流的影响。结果 :在钳制电压 (Vh) 10 5mV ,刺激电压 (Vt) 30mV条件下 ,神经元缺血 3min和 5min后持续钠电流显著增强。 3、10和 30μmol·L-1氟哌利多能明显抑制缺血引起的持续钠电流增强 (P <0 .0 1,n =7) ,不同浓度氟哌利多的作用间无显著差别。结论 :临床麻醉浓度的氟哌利多能够抑制体外脑缺血时海马神经元持续钠电流增强 ,对缺血神经元有保护作用。AIM: To study the effects of droperidol on the enhancement of persistent sodium currents induced by in vitro ischemia-like condition in isolated rat CA1 paramidal neurons. METHODS: Whole-cell patch-clamp recordings were made from enzymatically isolated rat CA1 hippocampal paramidal neurons. Ischemia was induced by oxygen and glucose deprivation. RESULTS: All of 3, 10 ,and 30 μmol·L -1 of droperidol significantly inhibited the enhancement of persistent sodium currents induced by ischemia, but the different concentrations did not show significant difference. CONCLUSION: Droperidol in clinical concentration can inhibit the enhancement of persistent sodium current induced by ischemia.

关 键 词：药效学 氟哌利多 持续钠电流 膜片钳 海马 缺血 

分 类 号：R973.3[医药卫生—药品] R965[医药卫生—药学]