鳗弧菌金属蛋白酶单克隆抗体的制备及鉴定  

Preparation and Identification of Monoclonal Antibodies of Metalloprotease of Vibrio anguillarum

作　　者：陈师勇[1] 张培军[1] 莫照兰[1] 张振冬[1] 邹玉霞[1] 徐永立[1] 

机构地区：[1]中国科学院海洋研究所海洋生物学重点实验室,青岛266071

出　　处：《高技术通讯》2003年第2期102-104,共3页Chinese High Technology Letters

基　　金：973规划 (G19990 12 0 0 3)资助项目。

摘　　要：鳗弧菌M 3胞外产物经纯化得到分子量为 36 5kDa的金属蛋白酶 ,浓缩后作为抗原免疫BALB/C小鼠。经细胞融合及筛选 ,得到三株稳定分泌抗金属蛋白酶的杂交瘤细胞株 ,分别命名为P2B1、P4A3和P4B1。亚型鉴定结果表明 ,三株细胞株产生的单抗均为IgG3型。蛋白质印迹 (WesternBlot)分析显示 ,所得到的单抗只与分子量为 36 5kDa的金属蛋白酶反应 ,具有较高特异性。三株单抗均能有效抑制蛋白酶活性。A 36.5 kDa metalloprotease was purified from the extracellular products(ECP) of Vibrio anguillarum M3, and used to immunize ALB/C mouse. Three monoclonal antibodies(MAbs), P2B1, P4A3 and P4B1, against the purified metalloprotease have been obtained and analyzed by Immuno-Western-Blot. The result showed that all of the three MAbs only reacted with the 36.5 kDa metalloprotease and neutratized its activity. Their isotypes were all characterized as IgG3. These data indicated that the MAbs could be useful tools to study the function of metalloprotease in the Vibrio anguillarum M3 infection.

关 键 词：制备 鉴定 鳗弧菌 金属蛋白酶 单克隆抗体 鱼 流行性疾病 

分 类 号：S941.41[农业科学—水产养殖]