东亚钳蝎毒抗癌多肽抗肿瘤作用的体内试验  

Antitumor effect of ant icancer polypeptide from Buthus Martensii Venom:experiment in vivo

作　　者：王秀丽 李冬晖 高春芳 沈东海 王平 赵光 

机构地区：[1]150医院全军肛肠外科中心,河南洛阳471031

出　　处：《实用医药杂志》2003年第2期124-125,共2页Practical Journal of Medicine & Pharmacy

摘　　要：目的研究东亚钳蝎毒抗癌多肽(anticancerpolypeptidefromButhusMartensiiVenom,APBMV)的细胞毒作用和体内抗癌作用。方法体外试验用噻唑蓝还原法(MTT法)检测APBMV对体外培养的人结肠癌细胞(Lovo,HT-29)、人胃癌细胞(SGC-7901)的生长抑制作用。建立小鼠移植瘤模型,观察APBMV腹腔注射以及口服给药对S-180肉瘤的体内抑瘤作用。结果体外试验表明,APBMV对Lovo、SGC-7901和HT-29细胞的半数抑制浓度(IC50)依次为60、71、139μg/ml。体内试验表明,APBMV9、6、3mg/kg,腹腔注射作用10d后,对小鼠S-180肉瘤的平均抑瘤率依次为64%、53%和47%,与对照组相比有统计学意义。结论APBMV对多种人消化道肿瘤细胞有较强的生长抑制作用,并对小鼠移植癌具有抑制作用。Objective To investigate cytotoxicity and antitumor eff ec t in vivo of anticancer polypeptide from Buths Martensii Venom(APBMV).Methods MTT assay was used to examine the growth inhibition of APBMV on human colon ca rcinoma cell line (Lovo and HT-29),human stomach carcinoma cell line(SG C-7901).The model of mice S-180sarcoma were used for in vivo antitum or test.Results The IC 50 of APBMV on Lovo cell,SGC-7901and HT-29cell were60,71,and13 9μg /ml respectively.Under the doses of9,6,and3mg /kg ip,qd×10d, the average tumor inhibitory rates of APBMV to mice S-180sarcoma were64% ,53%,and47%respectively(P<0.05).Conclusion APBMV would possess c ell growth inhibition to various human tumor cells in vitro and antitumor effect in vivo.

关 键 词：APBMV 人癌细胞株 细胞毒作用 抗肿瘤作用 东亚钳蝎毒抗癌多肽 

分 类 号：R979.1[医药卫生—药品] R961[医药卫生—药学]