静脉药瘾者人类免疫缺陷病毒核酸测定及亚型分析  

Detection of HIV nucleic acid and subtype analysis in 15 intravenous drug abusers

作　　者：吴南屏[1] 刘克洲[1] 陈智[1] Dennin.DH 

机构地区：[1]浙江大学医学院附一医院传染病研究所,杭州310003 [2]德国吕贝克大学

出　　处：《中华传染病杂志》2003年第1期14-16,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目 ( 39780 0 36 )

摘　　要：目的　了解静脉药瘾者人类免疫缺陷病毒 (HIV)感染情况及感染亚型 ,追踪传染源 ,预测未来流行株。方法　对 1997年从新疆至杭州的 15例静脉药瘾者血清进行抗 HIV的初步筛选 ,结果为阳性者再行免疫印迹 (Westernblot)、套式聚合酶链反应 (Nest PCR)及HIV核酸序列分析。结果15例新疆籍静脉药瘾者均感染了HIV 1,其中有 2例为E亚型 ,其余均为C亚型。结论　静脉吸毒者主要感染HIV 1C亚型。我国HIV 1主要流行株可能已经发生变化。Objective To investigate the molecular epidemiology as well as the subtypes of HIV in intravenous drug abusers, which can help pursue the source of infection in Zhejiang province and predict the epidemic strain in the future. Methods Such as ELISA, Western blot, nest PCR, DNA and sequencing technologies were used to analyze HIV subtypes of 15 strains isolated from intravenous drug abusers(IVDU) from Xinjiang autonomous region. Results All of the 15 IVDUs were infected with HIV 1, 2 of which were infected by subtype E and the others were infected by subtype C. Conclusions Subtype C is main HIV subtype infecting IVDUs. The epidemic subtype may have changed in China.

关 键 词：静脉药瘾者 人类免疫缺陷病毒 核酸序列分析 亚型 HIV感染 HIV-1 

分 类 号：R446.5[医药卫生—诊断学] R[医药卫生—临床医学]