HPLC法同时测定复方氧氟沙星滴耳液中氧氟沙星和地塞米松磷酸钠的含量  

Simultaneous Determination of Ofloxacin and Dexamethasone Sodium Phosphate in Compound Fufangyangfushaxin Ear Drops by HPLC

作　　者：祝辉[1] 钟元高[1] 罗自力[1] 何德云[1] 张国庆[1] 杜建红[1] 

机构地区：[1]成都军区药品仪器检验所,四川成都610017

出　　处：《解放军药学学报》2015年第5期390-392,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法同时测定复方氧氟沙星滴耳液中氧氟沙星和地塞米松磷酸钠的含量。方法色谱柱:Wonda Sil C18-WR(4.6 mm×250 mm,5μm);流动相:三乙胺溶液为流动相A(取三乙胺7.5 ml,加水稀释到1000 ml,用磷酸调p H至3.0±0.05),甲醇为流动相B;乙腈为流动相C,梯度洗脱;流速:1.0 ml·min-1;检测波长:242 nm;柱温:35℃。结果氧氟沙星在0.03~1.23μg(r=0.9999),地塞米松磷酸钠在0.02~0.88μg(r=1.0000)范围内与峰面积均呈良好的线性关系;氧氟沙星、地塞米松磷酸钠平均回收率分别为100.3%、98.6%。结论本文建立的HPLC方法操作简便,结果准确可靠。Objective To establish an HPLC method for simultaneous determination of ofloxacin and dexamethasone sodium phosphate in compound Fufangyangfushaxin ear drops. Methods Wonda Sil C18-WR( 4. 6 mm ×250 mm,5 μm) was used with gradient elution. Mobile phase A consisted of triethylamine solution( 7. 5 ml triethylamine,diluted with water to 1000 ml,p H adjuste to 3. 0 ± 0. 05 by phosphoric acid),mobile phase B consisted of methanol and mobile phase C consisted of methanol acetonitrile. The flow rate was 1. 0 ml·min-1,the detection wavelength was 242 nm and the column temperature was 35 ℃. Results The linear response ranged from 0. 03 to1. 23 μg for ofloxacin( r = 0. 9999) and 0. 02 to 0. 88 μg for dexamethasone sodium phosphate( r = 1. 0000),respectively. The average recovery of ofloxacin and dexamethasone sodium phosphate was 100. 3% and 98. 6%,respectively. Conclusion This HPLC method is simple,accurate and reliable.

关 键 词：复方氧氟沙星滴耳液 氧氟沙星 地塞米松磷酸钠 HPLC 

分 类 号：R927.2[医药卫生—药学] O657.72[理学—分析化学]