兔血管平滑肌细胞和聚羟基丁酯(PHB)的细胞相容性实验研究  

Experimental Studies on Cytocompatibility of Rabbit Vascular Smooth Muscle Cells and Polyhydroxybutyrate

作　　者：袁文周[1] 艾玉峰[1] 熊猛[1] 夏文森[1] 张琳西[1] 董兆麟[2] 杨清芳[3] 

机构地区：[1]第四军医大学西京医院整形外科中心,西安710032 [2]西北大学,西安710000 [3]西北工业大学,西安710071

出　　处：《科学技术与工程》2003年第1期42-46,共5页Science Technology and Engineering

基　　金：国家自然科学基金(30070777)

摘　　要：探索血管平滑肌细胞和新型可降解材料聚羟基丁酯(PHB)的细胞相容性,为组织人工血管的构建寻找理想的支架材料。将组织块法体外培养的兔血管平滑肌细胞种植在PHB膜片和PHB三维微孔支架上,在相差显微镜下观察细胞的粘附和生长情况,用MTT法测定细胞粘附率和细胞增殖指数,复合培养7d后进行扫描电镜观察并用流式细胞仪(FCM)测定细胞周期、DNA指数。结果兔血管平滑肌细胞在PHB膜片上粘附率为77％,细胞增殖符合细胞的生长曲线,在PHB三维微孔支架上生长情况良好,并被证实为二倍体细胞。结论是兔血管平滑肌细胞和聚羟基丁酯(PHB)的细胞相容性较好,但细胞与材料间的粘附有待进一步改善。To study the cytocompatibility of Polyhydroxybutyrate (PHB)combined with vascular smooth muscle cells (VSMCs) in vessel tissue engi-neering. VSMCs were cultured with PHB, then compared with PGLA and con-trol, the morphological characters were observed with SEM, cell adhere rateand cell proliferation were detected with MTT assay and cell cycle was de-tected with Flow Cytometer(FCM). The adhere rate of VSMCs which is seed-ed on PHB with 77%, when compared with PGLA and Control, PHB is less,but the cells seened on PHB grew well and are confirmed as diploid.It is con-clused that the results show that PHB has a good Cytocompatibility withVSMCs,but the adhere degree between Cell and material should be further im-proved.

关 键 词：实验研究 兔 血管平滑肌细胞 聚羟基丁酯 细胞相容性 组织工程 人工血管 可降解材料 

分 类 号：R318.11[医药卫生—生物医学工程]