利福霉素产生菌诱变育种研究  

Breeding of Rifamycin-Producing Strains with UV and Ion Beam Implantation

作　　者：王付转[1] 梁秋霞[1] 李宗伟[1] 王雁萍[1] 吴健[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052

出　　处：《郑州大学学报（理学版）》2003年第1期43-45,共3页Journal of Zhengzhou University:Natural Science Edition

摘　　要：用离子束及离子束和紫外复合因子诱变处理利福霉素产生菌 ,得到比对照菌株发酵效价提高 10 %以上的菌株共 13株 ,其中提高幅度最大可达 4 2 .70 % ,产生高产株最多的诱变剂量为 3× 10 1 4/ cm2 N+ ,而产量最高菌株的诱变剂量是 7× 10 1 5/ cm2 N+ .产生高产株较多的诱变剂量并不一定就是产生增产幅度最大菌株的诱变剂量 .The thirteen strains are obtained by using ion beam and ion beam together with UV implantation. Their potencies are over 10% and the highest is 42.7%, comparing with the original strain. The implanting dose to produce the most number of the higher potency strains is 3×10 14/cm 2N +, but the dose to produce the highest potency strain is 7×10 15/cm 2N +. So the dose to produce the most munber of the higher potency strains might not be the dose to produce the highest potency strain.

关 键 词：诱变育种 紫外线 离子束 利福霉素 诱变剂 安莎霉素类抗生素 发酵生产 高产菌株 

分 类 号：TQ465.9[化学工程—制药化工] TQ460.38