甲基对硫磷降解菌DLL-1的诱变育种  

MUTATION BREEDING OF METHYL PARATHION DEGRADING STRAIN DLL-1( PSEUDOMONAS sp.)

作　　者：刘智[1] 李顺鹏[1] 

机构地区：[1]南京农业大学资源与环境学院微生物学系,南京210095

出　　处：《土壤学报》2003年第2期293-300,共8页Acta Pedologica Sinica

基　　金：农业部"九五"重点项目 (编号 :96环 0 2 -0 2 )资助

摘　　要：采用紫外线和氯化锂诱变 ,从甲基对硫磷降解菌假单胞菌 (Pseudomonassp.)DLL -1中获取高效突变株DLL -E1 ,DLL -E2 ,DLL -E3 ,DLL -E4。并对其降解甲基对硫磷性能、降解对硝基苯酚性能、底物广谱性及胞外酶及粗酶液活性等生物学功能进行了研究。与出发菌株相比 ,上述性能均有所提高。选择DLL -E4作为进一步研究的对象 ,详细研究了其对甲基对硫磷及对硝基苯酚的耐受和降解情况。高效菌株的选育为农药残留降解的酶学研究打下了基础。Summary Four mutants (DLL-E1, DLL-E2, DLL-E3 and DLL-E4) were obtained by UV and LiCl mutation breeding of Methyl Parathion degrading strain Pseudomonas sp . DLL-1. Studies on their abilities to degrade Methyl Parathion and p nitrophenol, substrates, activities of ectoenzyme and crude enzyme showed that all mutants have higher biological activities compared to the original strain. Further studies on resistance and degradation of Methyl Parathion and p nitrophenol by DLL-E4 was conducted.

关 键 词：甲基对硫磷 降解菌 DLL-1 诱变育种 假单胞菌 突变株 生物学功能 

分 类 号：S154.39[农业科学—土壤学]