产毒性和致病性大肠埃希菌中小肠结肠炎耶尔森菌强毒力岛分布的研究  

Study on the prevalance of the "high pathogenicity island" of Yersinia enterocolitica WA in Enterotoxigenic,Enteropathogenic and Enteroaggregative E.coli strains

作　　者：王勇[1] 王红[2] 向前[2] 孙素霞[2] 俞守义 

机构地区：[1]解放军总后勤部卫生防疫队疾病监控科,北京100039 [2]第一军医大学流行病教研室 [3]热带医学研究所

出　　处：《中华流行病学杂志》2003年第3期213-215,共3页Chinese Journal of Epidemiology

基　　金：国家重点基础研究发展规划资助项目 (NO .G19990 5 410 1)

摘　　要：目的　了解小肠结肠炎耶尔森菌强毒力岛在产毒性 (ETEC)和致病性大肠埃希菌(EPEC)中的分布。方法　用聚合酶链反应扩增和原位杂交方法。结果　在检测的 93株ETEC和 10株EPEC的毒力岛的检出率分别为 3 2 .2 5 % ( 3 2 93 )和 3 0 .0 0 % ( 3 10 ) ,1株肠聚集性大肠埃希菌(EAggEC)也检出了毒力岛 ,而且这些阳性菌株中的毒力岛大部分连接到天门冬氨酸tRNA(asntRNA)位点。结论　ETEC、EPEC以及EAggEC是致病性较强的病原菌 ,而小肠结肠炎耶尔森菌强毒力岛在ETEC和EPEC中具有阳性率较高的分布 。Objective To detect the 'high pathogenicity island' of Yersinia enterocolitica WA in E.coli and the to provide evidence for theory base of bacterial evolution peocess and the different structures in different E.coil. Methods Polymease chain reaction (PCR),nucleic acid hybridization in situ were used to detect and identify HPI. DNA sequencing was used to compare the gene homology of HPI among E.coli with Yersinia enterocolitica (Yen). Results The irp2 and fyua genes of Yen HPI were investigated in E.coli strains. Among them,30 strains were isolated from 93 Enterotoxigenic E.coli (ETEC) strains and 3 strains were positive in 10 strains Enteropathogenic (EPEC). HPI was also detected in Enteroaggregative E.coli (EAggEC) strain. In most of these isolates, HPI was bordered by an asntRNA locus,as in Yersinia sp. Through sequential comparasion,the gene sequence homology was higher between in EPEC and EAggEC than ETEC and Yersinia enterocolica. Conclusions ETEC,EPEC and EAggEC were pathogenicity bactericas and many of them haboring HPI of Yen and the HPI had the same position in E.coli chromosome as Yersinia enterocolitica but the diversity of structure and sequence in these E.coli might suggest that the HPI of these different serotype E.coli were from different ancient bacterias. At the same time, the high positivty rate of HPI in E.coli might be crucial to virulence change, virulence evolution and virulence regulation in E.coli.

关 键 词：产毒性 致病性 大肠埃希菌 小肠结肠炎 耶尔森菌 强毒力岛 

分 类 号：R378[医药卫生—病原生物学]