丹参对抗缺氧/复氧引起的大鼠心室肌细胞收缩和细胞内钙的改变  

Salvia miltiorrhiza antagonized the alterations of contraction and intracellular calcium of rat ventricular cardiomyocytes induced by anoxia and reoxygenation

作　　者：徐万红[1] 曹春梅[1] 叶治国[1] 沈岳良[1] 蒋惠娣[2] 夏强[1] 

机构地区：[1]浙江大学医学院生理学与病理生理学研究所,浙江杭州310031 [2]浙江大学医学院浙江大学药学院,浙江杭州310031

出　　处：《中国病理生理杂志》2003年第3期348-352,共5页Chinese Journal of Pathophysiology

基　　金：浙江省中医药局重点资助项目 (No .G2 0 0 10 3 5 8)

摘　　要：目的 :观察丹参在常氧和缺氧 /复氧过程中对心肌细胞收缩和电刺激诱导的细胞内钙 ([Ca2 + ]i)瞬态的影响。方法 :采用酶解分离成年大鼠心室肌细胞化学缺氧模型 ,用视频跟踪计算机系统和细胞内双波长钙荧光系统分别观察心肌细胞收缩力学和 [Ca2 + ]i 等指标。结果 :丹参 (1- 9g/L)处理后降低心肌细胞最大收缩和舒张速率、收缩幅度以及电刺激诱导的 [Ca2 + ]i幅度 ,且呈剂量依赖性。缺氧后 ,与对照相比细胞收缩力和钙瞬态幅度降低、舒张末细胞长度缩短、舒张末钙水平增高 ;复氧后细胞收缩力、钙瞬态幅度和舒张末钙水平有所回复 ,但不能达对照水平。用 3g/L的丹参处理后 ,缺氧 /复氧引起的心肌细胞最大收缩和舒张速率、收缩幅度和电刺激诱导的 [Ca2 + ]i幅度高于单纯缺氧组 ,舒张末 [Ca2 + ]i水平低于单纯缺氧组。结论 :丹参可对抗缺氧AIM: To study the effect of salvia miltiorrhiza (SM) on cell contraction and intracellular calcium of enzymatically isolated rat ventricular myocytes during normoxia and anoxia/reoxygenation. METHODS: Contraction and intracellular calcium were determined with video tracking system and spectrofluorometric method, and the chemical anoxic method was employed. RESULTS: The ±d L /d t max , dL of cell contraction and the amplitude of [Ca 2+ ] i in the cardiomyocytes following SM treatment were decreased in a dose-dependent manner. During anoxia, the ±d L /d t max , dL and amplitude of [Ca 2+ ] i were decreased, while the diastolic Ca 2+ level was elevated compared with control group. All the contractile parameters and the diastolic Ca 2+ level were back toward pretreatment values during reoxygenation, but could not return to control level. After the treatment with SM (3 g/L), ±d L /d t max and dL of cell contraction and the amplitude of [Ca 2+ ] i were higher and the diastolic Ca 2+ level was lower than those in anoxia/reoxygenation group. CONCLUSION: SM antagonized effects of anoxia and reoxygenation on cell contraction and intracellular calcium in isolated ventricular myocytes.

关 键 词：丹参 大鼠 心室肌细胞收缩 细胞内钙 

分 类 号：R285.5[医药卫生—中药学]