一氧化氮抑制剂对腹膜透析大鼠腹膜形态及表面层的影响  

Effect of nitric oxide inhibitor on peritoneal structure and surfactant in rats with peritoneal dialysis

作　　者：黄胜[1] 叶任高[1] 彭文兴[1] 李晓艳[1] 段文娟[1] 吴金浪[1] 

机构地区：[1]中山大学附属第一医院肾内科,教育部肾脏病临床研究重点实验室,广东省肾脏病重点实验室,广东广州510080

出　　处：《中国病理生理杂志》2003年第3期370-373,T007,共5页Chinese Journal of Pathophysiology

基　　金：卫生部 2 11工程重点科研资助项目 (No .9815 1)

摘　　要：目的 :探讨高糖透析液对大鼠腹膜巨噬细胞一氧化氮 (NO)的产生、诱导型一氧化氮合酶 (iNOS)的表达和腹膜透析通透性的影响以及NO抑制剂的保护作用。方法 :培养生理盐水、1 5 %和 4 2 5 %葡萄糖透析液透析下的大鼠腹腔内的巨噬细胞 ,检测细胞培养上清液NO的浓度和巨噬细胞iNOS的表达 ,并观察 1 5 %及 4 2 5 %葡萄糖透析液和NO抑制剂透析下大鼠的液体超滤量和腹膜形态的变化。结果 :大鼠腹腔内巨噬细胞NO的产生随透析液中葡萄糖含量增加而增加 ;腹腔巨噬细胞iNOS的表达 ,葡萄糖透析组明显高于生理盐水组 ;NO抑制剂对光镜下腹膜形态无影响 ,但明显减轻 4 2 5 %葡萄糖透析液对超滤量和腹膜表面层的减少作用。结论 :一氧化氮抑制剂可明显改善高糖透析大鼠的腹膜通透性 。AIM: To study the products of nitric oxide and expression of iNOS in the peritoneal macrophages of rats during peritoneal dialysis, and the effect of nitric oxide inhibitor on the peritoneal structure and surfactant. METHODS: The peritoneal macrophages in the group dialysated by the normal salt water , 1.5%and 4 25%glucose water were collected and cultured, the product of nitric oxide and expression of iNOS in different groups were measured. The changes of peritoneum kinetics in rats after peritoneal dialysis for ten days with 1 5%, 4 25% dialysate and N:(G)-monomethyl-L-arginine (L-NMMA, 5 mg·kg -1 ·d -1 ) adding to the dialysate respective was also performed. The peritoneal structure and surfactant were studied by LM and EM. RESULTS: The products of nitric oxide in the rats dialysated by the normal water , 1 5% and 4 25% glucose water were (50 00±4 56) μmol/L, (83 00±2 34) μmol/L, (130 00±6 79) μmol/L, respectively The expression of iNOS by the peritoneal macrophages during peritoneal dialysis with glucose was higher than that during peritoneal dialysis with solid water. The water ultrafiltration in therats dialysised with 4 25% dialysate (32 00±0 23) mL was decreased compared to those dialysised with 1 5% dialysate (35 00±0 43) mL and in the control group(38 00±0 24) mL, P <0 05 The water ultrafiltration (34 00±0 14) mL and the solute transport was increased when adding the nitric oxide inhibitor to the dialysate( P <0 05). The effect of nitric oxide inhibitor on the peritoneal structure was not found, but the thickness of peritoneal surfactant and the ultrafiltration in two groups were (2 82±0 26) μm, and (1 12±0 34) μm, P <0 05. CONCLUSIONS: The level of nitric oxide was increased in rats during peritoneal dialysis. NO may affect the peritoneum permeability and the nitric oxide inhibitor adding to the dialysate improved the water ultrafiltration and the solute transport. The nitric oxide inhibitor decreased the damage

关 键 词：一氧化氮抑制剂 腹膜透析 巨噬细胞 大鼠 肾脏病 

分 类 号：R692[医药卫生—泌尿科学]