环磷酰胺在体外对大鼠肾小球系膜细胞增殖及凋亡的影响  

Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro

作　　者：谢彤[1] 吴平 唐德燊[1] 江黎明 

机构地区：[1]广东医学院肾脏病研究所,广东湛江524001 [2]广东医学院中心实验室,广东湛江524001

出　　处：《中国病理生理杂志》2003年第3期391-393,T008,共4页Chinese Journal of Pathophysiology

基　　金：广东医学院资助科研项目 (No.9911)

摘　　要：目的 :研究环磷酰胺 (CTX)在体外对大鼠肾小球系膜细胞 (GMC)增殖及细胞凋亡的影响。方法 :采用MTT法检测CTX、甲基强的松龙 (MP)、低分子肝素 (LMWH)对GMC细胞增殖的影响 ;采用光镜、荧光镜、流式细胞仪检测GMC细胞凋亡 ;GMC的Fas、Bcl- 2蛋白质水平采用免疫组化法检测。结果 :CTX、MP、LMWH均对GMC生长有抑制作用 ;CTX诱导GMC细胞凋亡 ,使GMC的Fas蛋白水平增加。结论 :CTX在体外可以抑制GMC细胞增殖 。AIM: To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS: GMC proliferation was detected by MTT method, GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis. The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS: The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION: CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.

关 键 词：环磷酰胺 大鼠 肾小球系膜细胞增殖 细胞凋亡 慢性增殖性肾小球肾炎 

分 类 号：R692.6[医药卫生—泌尿科学]