玉米转基因受体材料的筛选研究  

Genotype Slection for Genetic Transformation of Maize(Zea Mays L.)

作　　者：尚丽霞[1] 于志晶[1] 蔡勤安[1] 孟凡钢[1] 曾军[1] 林秀峰[1] 马瑞[1] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033

出　　处：《吉林农业科学》2015年第6期34-37,共4页Journal of　Jilin Agricultural Sciences

基　　金：吉林省科技发展计划项目(20130206005NY);农业部转基因专项(2014ZX08004-002)

摘　　要：玉米胚性愈伤组织的诱导率直接影响玉米遗传转化效率。本研究利用N6培养基诱导玉米幼胚产生胚性愈伤组织,以筛选玉米转基因受体材料。从215个玉米基因型中筛选出28个胚性愈伤组织诱导率较高的材料,其中,6个基因型的诱导率高于对照A188和Hi-II。基因型"吉V130"胚性愈伤组织诱导率最高,作为受体材料进行转基因研究中,获得了较高的转化效率。该基因型在今后玉米基因功能研究和基因工程育种中可作为重要的受体材料。The genetic transformation rate of maize is directly affected by induction rate of embryogenic callus-es. Friable embryogenic calluses were induced from immature embryos of maize on N6 induction medium and 28 genotypes with high induction efficiencies were screened from 215 maize inbred lines. The induction efficiencies of 6 genotypes were higher than that of control ‘A188' and ‘Hi-II' . The genotype ‘JiV130' confirmed in later experi-ment is the suitable genotype for genetic transformation, and can be routinely used for the production of large num-bers of transgenic plants. This result will be favorable for maize breeding by genetic engineering.

关 键 词：玉米基因型 胚性愈伤组织 转基因 

分 类 号：S513[农业科学—作物学]