近交系豫医无毛小鼠G显带带型分析及模式图  

Analysis on the G-banding pattern of the chromosomes and idiogram in inbred strain Yuyi hairless mice

作　　者：王纯耀[1] 宋国英[2] 杜献堂[3] 薛敬礼[1] 杜春燕[1] 邢金山[1] 祝庆蕃[1] 

机构地区：[1]河南省实验动物中心,郑州450052 [2]郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州450052 [3]郑州大学基础医学院微生物学与免疫学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第2期187-190,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目39970199;河南省医学科技创新人才工程资助项目2 0 0 10 90 7

摘　　要：目的 :观察近交系豫医无毛小鼠染色体G显带 ,并绘制出其染色体G带带型模式图。方法 :取近交系豫医无毛小鼠骨髓细胞制备染色体标本片 ,以胰酶法获取分散充分 ,带型清晰的早中期及中期分裂相。结果 :同一个中期细胞中染色体的带没有Nesbitt模式图的带多 ,不同细胞中带最多的染色体 ,其带的数目和位置与Nesbitt模式图基本一致。在核型分析的基础上绘制了近交系豫医无毛小鼠G带模式图 ,描述了各号染色体G带带型特征。结论 :近交系豫医无毛小鼠G带模式图的绘制 。Aim:To study the G banding pattern of the chromosomes and idiogram in inbred strain Yuyi hairless mice. Methods: Bone marrow cells from Yuyi hairless mice were obtained for chromosome analysis. The specimens were banded by Giemsa staining following trysinization which produced well scattered and sharply banded mitotic figures ranging from early metaphase to mid metaphase. Results: We had not found as many bands in one metaphase chromosome banded with trypsin Giemsa technique as that with Nesbitt. The banding numbers and positions for each well banding chromosome picked out from different metaphase plates were consistent with those of Nesbitt's idiogram. Idiogram of G banding patterns were constructed on the basis of karyotype analysis. The features of banding patterns of the individual chromosome were presented. Conclusion: The G banding karyotype idiogram of inbred strain Yuyi hairless mice could be used to study cell genetics of hairless mice.

关 键 词：近交系 小鼠 G显带带型 细胞遗传学 染色体 

分 类 号：Q343.2[生物学—遗传学]