HLC近交系小鼠骨髓嗜多染红细胞微核检测  

Determination of micronuleus of bone marrow polychromatic erythroblasts in HLC inbred strain mice

作　　者：王纯耀[1] 宋国英[2] 杜春燕[1] 薛敬礼[1] 李洪涛[1] 章金涛[1] 阎红霞[1] 樊波[1] 祝庆蕃[1] 

机构地区：[1]河南省实验动物中心,郑州450052 [2]郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第2期191-192,共2页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技创新人才工程资助项目2 0 0 10 90 7

摘　　要：目的 :了解HLC近交系小鼠骨髓微核自发率。方法 :将HLC小鼠、无毛小鼠及昆明种小鼠各分为 2组 ,一组取骨髓涂片观察 ,另一组腹腔注射 1 0 0mg/kg环磷酰胺 ,1 8h后取骨髓涂片观察 ,计算微核率。结果 :HLC近交系小鼠嗜多染红细胞微核自发率与无毛小鼠和昆明小鼠差异无统计学意义 (P >0 .0 5) ;腹腔注射环磷酰胺后 ,HLC近交系小鼠与无毛小鼠及昆明小鼠骨髓微核率均显著升高 (P <0 .0 1 ) ,但 3者之间差异无统计学意义 (P >0 .0 5)。结论 :HLC近交系与无毛小鼠及昆明小鼠微核自发率相似 。Aim:To research the micronuleus rate of bone marrow polychromatic erythroblasts (PCE) in HLC inbred strain mice. Methods:The HLC mice,hairless mice,and Kunming mice were allocated into 6 groups (2 groups per stock), the micronucleus rate of the 3 control groups were researched by bone marrow PCE, and the other 3, groups were abdominally injected cyclophosphamide 100 mg/kg and the micronuleus rate was determined after 18 hours. Results: The micronucleus rate of HLC mice's PCE had no significant difference from those of hairless mice and Kunming mice ( P >0.05). The micronucleus rates of HLC mice, hairless mice,and Kunming mice were significantly higher after injecting cyclophosphamide ( P <0.01) , but there were no significant difference among them( P >0.05). Conclusion:The micronucleus rate of 3 stocks are similar.The sensitivity of the three stocks of mice to cyclophosphamide are similar.

关 键 词：HLC 近交系 小鼠 骨髓微核 微核率 

分 类 号：Q343[生物学—遗传学]