郑州地区汉族正常人群、糖尿病和糖尿病并发冠心病患者芳香酯酶Q192R基因多态性  

Relationship between arylesterase/paraoxonase1Q192R gene polymorphism and diabetes mellitus complicated with coronary artery disease in population of Han nationality of Zhengzhou

作　　者：钱书虹[1] 钱庆文[2] 王书瑾 王诗瑾[4] 

机构地区：[1]郑州大学检验系,郑州450052 [2]郑州大学第一附属医院内科,郑州450052 [3]郑州水工机械厂职工医院,郑州450052 [4]河南省临床检验中心,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第2期225-227,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关基金资助项目991170 75

摘　　要：目的 :研究郑州地区汉族正常人群、2型糖尿病 (DM)及DM并发冠心病 (DM CAD)患者芳香酯酶(Arylesterase/Paraoxonase ,ArE/PON1 ) 1 92位基因多态性。方法 :用比色法测定郑州地区汉族正常人群 (61例 )、DM(60例 )和DM CAD(67例 )患者血清ArE/PON1活性 ,用聚合酶链式反应分析技术检测ArE/PON1基因 1 92位多态性 ,用酶法和酶联免疫法分别测定血清胆固醇、高密度脂蛋白胆固醇 (HDLc)、氧化型低密度脂蛋白 (oxLDL)等指标。结果 :郑州地区汉族人群中存在有ArE/PON1 1 92位基因多态性 ,Q、R基因频率健康人群中分别为 0 .56与 0 .44 ;DM组为 0 .53与 0 .47;DM CAD组为 0 .38和 0 .62。DM CAD组R基因频率高于DM组和对照组。基因型百分比DM CAD组RR(31 % )高于DM组 (1 3 % )和对照组 (1 1 % ) (P <0 .0 5)。DM与DM CAD组血清ArE/PON1活性水平低于对照组 ,以DM CAD组为最低 (P <0 .0 5)。酶活性的降低与HDLc、HDL2C呈正相关 ,与oxLDL呈负相关。结论 :郑州地区汉族人群存在ArE/PON1 1 92位基因多态性 ,并提示R基因是郑州地区汉族人群DMAim:To investigate the relationship between arylesterase/paraoxonase(ArE/PON1) Q192R gene polymorphism and type 2 diabetes mellitus (type 2 DM) complicated with coronary artery disease (CAD) in population of Han nationality in Zhengzhou area.Methods:Serum ArE/PON1 activity and its Q192R gene polymorphism were determined in 60 patients with type 2 DM, 67 DM patients complicated with coronary artery disease(DM CAD),and 61 healthy subjects. ArE/PON1Q192R gene polymorphism were detected by PCR method.Results:The frequencies of the Q allele and R were 0.56 and 0.44 in healthy subjects,0.53 and 0.47 in patients with DM,0.38 and 0.62 in patients with DM CAD,respectively. The R gene frequency in DM CAD group was higher than that in DM group and healthy subjects. The percentage of RR genotype in DM CAD group (31%)was higher than that (13%) in DM group and that (11%)in healthy subjects.Serum ArE/PON1 activity in DM and DM CAD groups were lower than that in healthy subjects and it was the lowest in DM CAD group.There were a positive correlation between ArE/PON1 activity and HDLc,HDL2C,and a negative correlation between ArE/PON1 activity and oxLDL.Conclusion:ArE/PON1Q192R polymorphism exists in Zhengzhou population of Han nationality. It is suggested that R allele might be a risk factor for DM complicated with CAD in Zhengzhou population of Han nationality.

关 键 词：郑州地区 汉族正常人群 糖尿病 冠心病 芳香酯酶 Q192R 基因多态性 糖尿病并发症 

分 类 号：R587[医药卫生—内分泌] R541.4[医药卫生—内科学]