大鼠睾丸间质细胞分离及其鉴定  

The Isolation and Examination of Rat Testis Leydig Cell

作　　者：况海斌[1] 王新长[1] 方廉[1] 

机构地区：[1]江西医学院生理教研室生殖研究室,江西南昌330006

出　　处：《江西医学院学报》2002年第5期7-9,共3页Acta Academiae Medicinae Jiangxi

基　　金：江西省自然科学基金资助 (0 0 40 0 6 9)

摘　　要：目的和方法 :通过血管冲洗、Ⅱ型胶原酶消化以及Percoll梯度离心 ,建立大鼠睾丸间质细胞分离方法。结果 :(1)Percoll密度梯度离心后纯化的间质细胞 3β HSD染色阳性百分率可达 85 %。 (2 ) 1.2× 10 -3 IU/mlhCG可显著性增加间质细胞睾酮的分泌 (P <0 .0 5 )。结论Objective: To get the isolation of Leydig cell from rat testis.Methods:A multistep procedure including vascular perfusion,dissociation of type Ⅱ collagenase and Percoll gradient centrifugation was employed.Results:(1)The purified Leydig cell fraction after Percoll density-gradient centrifugation contained 85% well-preserved 3β-hydroxysteroid dehydrogenase(3β-HSD);(2)The secretion of testosterone on hCG-induced significantly increases in the Leydig cell isolated(P&lt;0.05).Conclusion:It suggests that the method is reliable and has somewhat applicant prospect.

关 键 词：睾丸间质细胞 人绒毛膜促性腺激素 PERCOLL 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] Q2[医药卫生—基础医学]