重组平颏海蛇磷脂酶A_2抑制晚期糖基化终产物诱导的大鼠血管平滑肌细胞增殖  

Recombinant Lapemis hardwickii phospholipase A_2 inhibits rat vascular smooth muscle cell proliferation stimulated by advanced glycation end products

作　　者：欧阳平[1] 许顶立[1] 杨文利[2] 赖文岩[1] 徐安龙[2] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515 [2]中山大学生命科学学院海洋生物功能基因组开放实验室,广东广州510275

出　　处：《第一军医大学学报》2003年第3期210-212,共3页Journal of First Military Medical University

基　　金：国家863计划(2001AA628090);广州市科委科技攻关项目(JB00000448165)~~

摘　　要：目的观察重组平颏海蛇磷脂酶A2(rPLA2)对晚期糖基化终产物(AGEs)刺激下离体大鼠胸主动脉血管平滑肌细胞增殖的影响。方法体外培养大鼠主动脉血管平滑肌细胞(VSMCs),应用不同浓度的AGEs分别刺激VSMCs并设立对照组进行比较,采用非放射性的MTS/PES法确定血管平滑肌细胞的增殖状态。结果与对照组相比,AGEs对VSM-Cs增殖具有明显的刺激作用(P<0.05)。在AGEs刺激下,rPLA2可明显抑制VSMCs的生长(0.753±0.098 vs. 1.100±0.226, P<0.05)。结论rPLA2可抑制晚期糖基化终产物诱导的血管平滑肌细胞增殖。Objective To observe the effects of recombinant Lapemis hardwickii phospholipase A 2 (rPLA 2 ) on vascular smooth muscle cell (VSMC) proliferation stimulated by advanced glycation end products (AGEs). Methods Rat aortic VSMCs were cultured in vitro and stimulated with AGEs of different concentrations prior to co-treatment with rPLA 2 and AGEs. The re-sponse of the VSMCs to these treatments was observed in comparison with that of the control group. Non-radioactive MTS/PES assay was adopted for the quantification of the cell proliferation ratio. Results Compared with the control group, AGEs significantly stimulated VSMCs proliferation (P<0.05), but even with AGEs stimulation, rPLA 2 inhibited the growth of VSMCs (0.753±0.098 vs1.100±0.226, P<0.05). Conclusion rPLA 2 can significantly inhibit the proliferation of VSMCs stim-ulated by AGEs.

关 键 词：血管平滑肌细胞 药物作用 重组平颏海蛇磷脂酶A2 晚期糖基化终产物 细胞增殖 

分 类 号：R540.2[医药卫生—心血管疾病]