瘢痕疙瘩及增生性瘢痕Fas蛋白表达的免疫组化分析  

Immunohistochemical analysis of Fas protein expression in hypertrophic scar and keloid

作　　者：鲁峰[1] 高建华[1] 黎小间[1] 

机构地区：[1]第一军医大学南方医院整形外科,广东广州510515

出　　处：《第一军医大学学报》2003年第3期228-229,232,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(39870807)~~

摘　　要：目的鉴别诊断瘢痕疙瘩及增生性瘢痕。方法应用免疫组化方法法检测Fas蛋白在增生性瘢痕、瘢痕疙瘩及其周围正常皮肤组织中的表达,并以图像定量分析比较其差异。结果瘢痕疙瘩的Fas蛋白表达显著高于增生性瘢痕;在瘢痕疙瘩及其周围正常皮肤中Fas蛋白表达均很高,两者之间无显著差异;而增生性瘢痕的Fas蛋白表达较低,且与周围正常皮肤之间差异显著。结论以免疫组化方法检测Fas蛋白来鉴别诊断瘢痕疙瘩及增生性瘢痕,方法简单,可进一步推广。Objective To find an efficient diagnostic method of hypertrophic scar and keloid. Methods Immuno-histochem-istry was employed to detect the expression and distribution of Fas protein in keloids, hypertrophic scars and their surrounding normal skins. Image analysis was performed to quantitatively compare the differences of the protein expression in these tis-sues. Results The expression level of Fas antigen was much higher in the keloids and their surrounding normal skins than in hypertrophic scars, and no significant difference in the expression level between the former 2 tissues was found. In hypertrophic scars, however, much lower Fas protein expression was detected in comparison with that in their surrounding normal skins(P<0.01). Conclusion Immunohistochemical detection of Fas protein is a simple method that is well applicable in the differ-ential diagnosis of pathological scars.

关 键 词：瘢痕疙瘩 诊断 FAS蛋白 免疫组织化学 

分 类 号：R619.6[医药卫生—外科学]