T型分叉流槽中前列环素和内皮素分泌的研究  

THE SECRETION OF PGI_2 AND ET-1IN T-BIFURCATION FLOW CHAMBER

作　　者：胡嘉[1] 胡江[1] 高宇欣[1] 陶祖莱[1] 

机构地区：[1]中国科学院力学研究所国家微重力实验室,北京100080

出　　处：《生物物理学报》2002年第4期452-455,共4页Acta Biophysica Sinica

基　　金：国家自然科学基金重点项目(19732003)

摘　　要：动脉粥样硬化(atherosclerosis)的非随机分布与当地的血流动力环境有关。借助平行平板式平直流槽和以T型分叉流槽为代表的平行平板式异型流槽,可以模拟血管的主要形状特征。首先,在数值模拟的基础上分析了流型特征参数,确定了流槽的设计尺寸。然后,通过实验研究,探讨流型改变对内皮细胞血管活性物质分泌的影响,发现扩张效应流线偏转和驻点效应使得异型流槽前列环素和内皮素的分泌水平与相同入口雷诺数(Re)条件下的平直流槽分别有降低趋势和显著差异。为进一步研究流型对血管内皮细胞的影响提供了实验数据。The non-random distribution of atherosclerosis was related to local hemodynamic environment. Using the mended parallel plate flow apparatus, such as the parallel plate rectangle and T-bifurcation flow chamber, and reciprocating syringe pump providing fluid flow, endothelial culture models were established in vitro with the same environment factors as steady culture. A numerical calculation procedure based on the finite volume method was employed to simulate flow fields in both flow chambers. The effect of flow pattern on vasoactive mediators secretion was studied. It was shown that at the same entrance Re number, the secretion of PGI 2 was decreased and the secretion of ET-1 was significantly decreased in T-Bifurcation flow chamber compared to uniform laminar shear stress in rectangle flow chamber during 8 h flow period. In conclusion, the spatial distribution of fluid shear stress influenced the local secretion of vasoactive mediators, such as PGI 2 and ET-1.

关 键 词：剪应力 流型 内皮细胞 前列环素 内皮素 

分 类 号：Q6[生物学—生物物理学]