S7核糖体蛋白基因序列变异与亚科鱼类的单系性研究  

STUDIES ON MONOPHYLY OF DANIONINE FISHES(TELEOSTEI: CYPRINIDAE),BASED ON SEQUENCE VARIATIONS OF THE S7 RIBOSOMAL PROTEIN GENE

作　　者：王绪桢[1] 何舜平[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》2003年第2期122-126,共5页Acta Hydrobiologica Sinica

基　　金：自然科学重点基金 ( 39830 0 5 0 );中国科学院重点项目 (K2 -92 5 - J1 0 44);国家重点基础发展规划项目 (G2 0 0 0 0 46 80 4)资助

摘　　要：使用分子生物学的方法对亚科鱼类的单系性进行了探讨。通过PCR方法 ,获得了 13种鲤科鱼类S7核糖体蛋白基因第 1内含子序列 ,其中包括 6种亚科鱼类。使用MEGA软件中的Neighbor Joining法和Most Parsimony法分别构建分支系统图。研究结果显示目前所确认的亚科鱼类实际上没有形成单系类群。其中属、波鱼属和低线属位于系统树基部 ,显示出原始性。而由细鲫属、马口鱼属和属构成的类群相对于亚科中的原始种类起源较晚 ,可能和较晚起源的东亚鲤科类群之间有更为密切的关系。The S7 ribosomal protein gene was used as a DNA marker for the phylogenetic analyses of Cyprinidae in present paper. Sequences of the 1 st intron of the S7 ribosomal protein gene were determined by Polymerase Chain Reaction (PCR) for 13 species of cyprinid including 6 danionine species. An alignment of 743 nucleotide sites was used in the phylogenetic studies. Out of these sites, variable sites were 592 and parsimony informative sites 366. Sequence divergence among the cyprinids analyzed ranges from 2.6% between close species to 48.0% between distant species. The cladograms were produced using Neighbor-Joining method and Most-Parsimony method included in software MEGA. The present results indicate that the currently recognized Danionine fishes cannot be related as a monophyletic group. The location of Danio, Rasbora and Barilius closely nearing the root of the cladograms shows that they seem to be derived earlier from common cyprinid ancestor. Compared with these basal Danioninae genera, speciation of the genera Aphyocypris, Zacco and Opsariichthys occurred more recently and they may be closely related to the East Asian fish group.

关 键 词：Dan亚科 S7核糖体蛋白基因 系统发育 鲤科 

分 类 号：Q959.14[生物学—动物学]