白细胞介素-2对大鼠心肌Ca^(2+)ATPase和Na^+/K^+ATPase的影响  

Effect of interleukin-2 on the activity of Ca^(2+) ATPase and Na^+/K^+ ATPase of sarcoplasmic reticulum and sarcolemma

作　　者：曹春梅[1] 夏强[1] 傅琛[1] 蒋惠娣[1] 叶治国[1] 沈岳良[1] 陈君柱[2] 

机构地区：[1]浙江大学医学院生理学教研室,杭州310031 [2]浙江大学医学院第一附属医院心内科,杭州310031

出　　处：《生理学报》2003年第1期83-90,共8页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNaturalScienceFoundationofZhejiangProvinceforTalents (No .RC990 38)

摘　　要：为了探讨IL 2对心肌细胞内钙影响的可能机制 ,用光学法检测心肌肌浆网Ca2 + ATPase的活性 ,以及细胞膜Ca2 + ATPase和Na+ /K+ ATPase的活性。结果 :(1)用IL 2 (10、40、2 0 0、80 0U/ml)灌流心脏后 ,其肌浆网Ca2 +ATPase的活性随IL 2浓度的升高而增强 ;(2 )在ATP浓度为 0 1～ 4mmol/L时 ,Ca2 + ATPase的活性随ATP浓度的升高而增强 ,由IL 2 (2 0 0U/ml)灌流后的心脏获得肌浆网 (SR) ,其Ca2 + ATPase的活性对ATP的反应强于对照组 ;(3 )在 [Ca2 + ]为 1～ 40 μmol/L时 ,心脏SRCa2 + ATPase的活性随 [Ca2 + ]增加而增强 ,而IL 2灌流心脏后分离的SR ,其Ca2 + ATPase活性在 [Ca2 + ]升高时没有明显改变 ;(4)用nor BNI (10nmol/L)预处理 5min后 ,IL 2 (2 0 0U/ml)灌流后不再使SRCa2 + ATPase的活性增强 ;(5 )用PTX (5mg/L)预处理后 ,IL 2对SRCa2 + ATPase的影响减弱 ;(6)用磷脂酶C (PLC)抑制剂U73 12 2 (5 μmol/L)处理后 ,IL 2不再使SRCa2 + ATPase活性增高 ;(7)用IL 2直接处理从正常大鼠分离的SR后 ,对SRCa2 + ATPase活性无明显影响 ;(8)IL 2灌流后 ,对心肌细胞膜Ca2 + ATPase和Na+ /K+ATPase活性没有显著影响。上述结果表明 ,IL 2灌流心脏后使心肌肌浆网Ca2 + ATPase的活性增加 ,心肌细胞膜上的κ 阿片受?The purpose of the present study was to investigate whether interleukin 2 ( IL 2 ) changes the activity of sarcoplasmic reticulum (SR) Ca 2+ ATPase , sarcolemmal Ca 2+ ATPase and Na +/K + ATPase by measuring the Pi liberated from ATP hydrolysis with colorimetrical methods. It was shown that the activity of Ca 2+ ATPase in SR from IL 2 perfused (10, 40, 200, 800 U/ml) rat heart increased dose dependently. After incubation of the SR with ATP (0.1～4 mmol/L), the activity of SR Ca 2+ ATPase increased dose dependently in the control group. In the SR from 200 U/ml IL 2 perfused hearts, the activity of Ca 2+ ATPase was much higher than that in the control group. On the other hand, incubation of the SR with Ca 2+ (1～40 μmol/L) increased the activity of SR Ca 2+ ATPase in the control group. The activity of SR Ca 2+ ATPase of IL 2 perfused hearts was inhibited as the function to Ca 2+ . Pretreatment with specific κ opioid receptor antagonist nor BNI (10 nmol/L) for 5 min attenuated the effect of IL 2 (200 U/ml) on the activity of SR Ca 2+ ATPase . After pretreatment with pertussis toxin (PTX, 5 mg/L) or U73122 (5 μmol/L), IL 2 failed to increase SR Ca 2+ ATPase activity. The activity of SR Ca 2+ ATPase was not changed by incubation of SR isolated from normal hearts with IL 2 . Perfusion of rat heart with IL 2 did not affect the activity of sarcolemmal Ca 2+ ATPase and Na +/K + ATPase . It is concluded that perfusion of rat heart with IL 2 increases the activity of SR Ca 2+ ATPase dose dependently, which is mainly mediated by cardiac κ opioid receptor pathway including a PTX sensitive Gi protein and phospholipase C. IL 2 increases the activity of SR Ca 2+ ATPase as the function to ATP, but inhibits the activity of SR Ca 2+ ATPase as the function to Ca 2+ . IL 2 has no effect on the activity of sarcolemma

关 键 词：生理学 白细胞介素2 分光光度法 心脏 肌浆网 Ca^2+转运ATP酶 阿片样受体 

分 类 号：R33[医药卫生—人体生理学]