极大螺旋藻(Spirulina maxima)和微小小球藻(Chlorella minutissima)对UV-B增强的响应  

The responses of Spirulina maxima and Chlorella minutissima to enchanced UV-B radiation

作　　者：陈善文[1] 韩志国[2] 武宝玕[3] 

机构地区：[1]汕头大学海洋生物研究所,汕头515063 [2]暨南大学水生生物研究所,广州510632 [3]暨南大学生物工程学系,广州510632

出　　处：《生态科学》2002年第4期294-298,共5页Ecological Science

摘　　要：本文研究了极大螺旋藻(Spirulina maxima)和微小小球藻(Chlorella minutissima)分别对紫外线-B(UV-B)的短时间和长时间处理的响应。在不同强度(0～2.0w·m^(-2))UV-B的短时间(6h)作用下,这两种藻的光化效率(F_V/F_M)均明显的降低,且降低的程度与UV-B强度呈正相关,极大螺旋藻较微小小球藻降低更显著;UV-B吸收物质的分泌和超氧化物歧化酶(SOD)活性,都与UV-B的增强无明显关系。经相同强度(0.9或1.8w·m^(-2))的UV-B长时间(1～80d)作用后,F_V/F_M随着作用时间的延长,极大螺旋藻的表现是先降后升,而微小小球藻则表现为逐渐下降;对于UV-B吸收物质的分泌和SOD酶活性,极大螺旋藻都表现为逐渐增强,而微小小球藻却表现为无明显变化。The responses of Spirulina maxima (blue-green alga) and Chlorella minutissima (green alga) to short-term (6 h) UV-B treatment and long-term (1-80 d) UV-B treatment were investigated using Chlorophyll (Chl) fluorescence, UV spectrophotometer and improved Fridovich method. After short-term treatment (6 h) under different UV-B intensity (0-2.0 w·m-2), FV/FM of the two species were both substantially inhibited, and that of S. maxima were inhibited more than that of C. minutissima. However, the secretion of UV-B absorbing substances and SOD activity in two species after short-time treatment were not related to UV-B. Under long-term treatment (1 -80 d) with the same UV-B intensity (0.9 or 1.8 w·m-2), FV/FM of S. maxima were decreased firstly followed by remarkable increasing, while that of C. minutissima were decreased gradually. Within the treatment time, the secretion of UV-B absorbing substances and SOD activity increased gradually in S. maxima, while there are no changes in C. minutissima.

关 键 词：螺旋藻 小球藻 UV-B 叶绿素荧光 超氧化物歧化酶 

分 类 号：Q178[生物学—水生生物学]