甜樱桃茎尖培养及PNRSV的RT-PCR检测  

In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSV by RT-PCR

作　　者：代红艳[1] 张志宏[1] 吴禄平[1] 侯义龙[1] 吕德国[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《园艺学报》2003年第1期87-89,共3页Acta Horticulturae Sinica

摘　　要：研究了茎尖大小、接种方式、培养基成分、试材基因型对甜樱桃品种茎尖培养的影响。 1年生成熟枝条上茎尖成苗率为 8.3%～ 2 3.7% ,嫩梢上的茎尖成苗率为 2 7.3%～ 37.5 %。利用RT PCR技术对部分甜樱桃试管苗进行了早期病毒鉴定 ,筛选出一些不带李坏死环斑病毒 (PNRSV)的甜樱桃试管苗 ,并证明甜樱桃试管苗微茎尖培养不能有效脱除PNRSV。The effects of size of inoculated shoot tip, method of inoculation, medium and genotype on shoot tip culture of sweet cherry ( Prunus avium L.) cultivars were studied. The rate of shoots forming from tips of one year old twigs was low, 8.3% to 23.7%, while the rate of shoots forming from tips of actively growing shoots was higher, 27.3% to 37.5%. Prunus necrotic ringspot virus (PNRSV) was detected early in some of sweet cherry plantlets in vitro with RT PCR technique. Plants free of PNRSV were found. It was proved that PNRSV could not be eliminated effectively from sweet cherry plantlets in vitro by culture of minor shoot tip.

关 键 词：PNRSV RT-PCR检测 甜樱桃 茎培养 李坏死环斑病毒 组织培养 试管苗 

分 类 号：S662.5[农业科学—果树学]