诱导黄瓜直接器官发生主要影响因素的研究  

Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro

作　　者：侯爱菊[1] 朱延明[1] 杨爱馥[1] 张彬彬[1] 

机构地区：[1]东北农业大学生命科学学院植物生物工程研究室,哈尔滨150030

出　　处：《园艺学报》2003年第1期101-103,共3页Acta Horticulturae Sinica

摘　　要：对诱导黄瓜直接器官发生的主要因素进行了探讨。结果表明 :外植体类型、基因型及植物生长调节剂对再生频率均有显著影响。子叶节是最佳的外植体类型 ;‘长春密刺’及‘龙杂黄七号’再生频率较高 ,分别达到 93.2 %和 92 .5 % ,前者每块外植体上的平均芽数 (13.2 )极显著高于后者 (3.1) ;6 BA单独存在即可诱导产生丛生芽 ,0 .The main factors influencing the frequency of direct organogenesis of cucumber( Cucumis sativus L.)were discussed.The results showed that the types of explants,genotypes and plant growth regulator had significantly effects on regenerating frequency.The optimum explant is cotyledonary nodes.Genotypes'Changchun mici'and'Longzahuang 7'had higher frequency of regeneration which were 93.2% and 92.1% respectively. But the former differentiated much more buds(13.2 per explant)than that of the latter(3.1 per explant). 6 BA can effectively induced buds and the optimum concentration is 0.5mg/L.

关 键 词：影响因素 黄瓜 再生体系 直接器官发生 芽分化 外植体 植物生长调节剂 基因型 

分 类 号：S642.2[农业科学—蔬菜学]