拳参的DNA裂解活性成分研究  

Studies on chemical constituents possessing DNA cleavage activity

作　　者：肖凯[1] 宣利江[1] 徐亚明[1] 白东鲁[1] 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所,上海200433

出　　处：《中草药》2003年第3期203-206,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究蓼科植物拳参的水溶性成分 ,并测试其 DNA裂解活性。方法　利用反相层析的方法进行分离纯化 ,根据化合物的化学性质与光谱数据鉴定其结构。结果　自拳参根茎的 6 0 %丙酮提取物中分得 10个化合物 ,确定其结构分别为没食子酸 ( ) ,色氨酸 ( ) ,2 ,6 -二羟基苯甲酸 ( ) ,( +) -儿茶素 ( ) ,绿原酸 ( ) ,( - ) -表儿茶素 - 5 - O-β- D-吡喃葡萄糖苷 ( ) ,( +) -儿茶素 - 7- O-β- D -吡喃葡萄糖苷 ( ) ,1- ( 3- O-β- D-吡喃葡萄糖基 - 4,5 -二羟基 -苯基 ) -乙酮 ( ) ,( +) -儿茶素 - 5 - O-β- D -吡喃葡萄糖苷 ( )和 ( - ) -表儿茶素 ( )。结论　化合物 , , ～ 为首次从该植物中分得 ,化合物 , , , , , 具有很强的 DNA裂解活性。Object To study the chemical structures and DNA cleavage activity of the water-soluble constituents from Polygonum bistorta L.Methods To isolate the constituents by reverse phase chromatography, and characterize their structures by the analysis of chemical property and spectral data.Results Ten compounds were isolated from the 60% acetone extract of the rhizoma from P.bistorta.Their structures were elucidated as gallic acid (Ⅰ), tryptophan (Ⅱ), 2, 6-dihydroy-bezoic acid (Ⅲ), (+)-catechin (Ⅳ), chlorogenic acid (Ⅴ), (-)-epicatechin-5-O-β-D-glucopyranoside (Ⅵ), (+)-catechin-7-O-β-D-glucopyranoside (Ⅶ), 1-(3-O-β-D-glucopyranosyl-4, 5-dihydroxy-phenyl)-ethanone (Ⅷ), (+)-catechin-5-O-β-D-glucopyranoside (Ⅸ) and (-)-epicatechin (Ⅹ), respectively.Conclusion Compounds Ⅱ, Ⅲ, Ⅴ-Ⅹ were isolated from the plant for the first time.Compounds Ⅰ, Ⅳ, Ⅵ, Ⅶ, Ⅸ, Ⅹ showed significant DNA cleavage activity.

关 键 词：拳参 水溶性成分 DNA裂解活性 

分 类 号：R284.1[医药卫生—中药学]