苦玄参、黄芩与黄柏的大孔树脂提取研究  

Study on extracting purified active components of Picria fel-terrae, Scutellaria baicalensis and Phellodendron chinense with macroporous resins

作　　者：邹节明[1] 陆浩[1] 何斌[1] 吴敏菊[1] 

机构地区：[1]桂林三金药业股份有限公司药物研究所,广西桂林541004

出　　处：《中草药》2003年第3期222-226,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的　探讨大孔吸附树脂在精制苦玄参、黄芩与黄柏有效部位的应用。方法　以中药中的有效成分为指标 ,筛选适用的大孔吸附树脂型号 ,评价树脂吸附与解吸工艺。结果　苦玄参、黄芩与黄柏提取物精制适用树脂型号分别为 HPD5 0 0、HPD30 0与 HPD10 0 ,吸附阶段的泄漏点分别为 1.5 ,0 .5和 1;饱和点分别为 11,2和 4.75 ;解吸阶段的适用乙醇浓度分别为 5 0 %,30 %和 5 0 %。结论　不同型号的大孔吸附树脂对中药有效部位的提取精制有较大差别。Object To study the application of macroporous resins to the purified active components of Picria fel-terrae Lour., Scutellaria baicalensis Georgi, and Phellodendron chinense Schneid. Methods With the purified active components of Chinese materia medica (CMM) as a standard, we selected the suitable macroporous resins and studied the optimum technological parameters of the adsorption and elution. Results The suitable three types of macroporous resins which were used to the purified active components of CMM were HPD500 for P. fel-terrae., HPD300 for S. baicalensis, and HPD100 for P. chinense. In the absorption course, when the ratios between the medicinal materials amount and the volume of macroporous resins were 1.5 for P. fel-terrae, 0.5 for S. baicalensis and 1 for P. chinense, the purified active components of CMM appeared leaking, and were no more absorbed when the ratios were 11 for P. fel-terrae, 2 for S. baicalensis and 4.75 for P. chinense. In the elution course, the optimum alcohol concentrations were 50% for P. fel-terrae, 30% for S. baicalensis and 50% for P. chinense. Conclusion It is obviously different to refine the active components of CMM, while using the diverse types of macroporous resins.

关 键 词：苦玄参 苦玄参苷IA 黄芩 黄芩苷 黄柏 盐酸小檗碱 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]