不同来源桑白皮东莨菪内酯含量测定  

Determination of scopoletin in Cortex Mori from different sources

作　　者：孙静芸[1] 周羽琪[1] 李洪玉[1] 章建民[1] 

机构地区：[1]浙江省中医药研究院,浙江杭州310007

出　　处：《中草药》2003年第3期266-268,共3页Chinese Traditional and Herbal Drugs

基　　金："九五"国家重点科技攻关项目 ( 96 -90 3-0 2 -0 3)

摘　　要：目的　研究不同来源桑白皮东莨菪内酯含量和测定方法。方法　采用 HPL C法 ,以 Me OH- H2 O- HAc( 40∶ 6 0∶ 0 .2 5 )为流动相 ,345 nm为检测波长。结果　东莨菪内酯平均回收率为 98.88%,RSD为 1.91%,线性范围为 0 .0 2 5 6～ 0 .12 8μg。不同来源最高含量为 0 .0 173%,最低含量为 0 .0 0 2 %。结论　 HPL C测定法简便 ,稳定可靠 ,重复性好 ,灵敏度高。Object To study the contents and the determinating method of scopoletin in Cortex Mori from different sources.Methods Scopoletin was determined by HPLC.The mobile phase was MeOH-H 2O-HAc (40∶60∶0.25).Detection wavelength was at 354 nm.Results The average recovery was 98.88% with RSD=1.91% and the linear range was 0.025 6—0.128 μg.The highest content of scopoletin was 0.017 3% and the lowest content was 0.002% from different producing areas.Conclusion The method is easy, accurate, reliable with good reproducibility and high sensitivity.

关 键 词：桑白皮 东莨菪内酯 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]