长春花黄化植原体(PY)株系的检测与鉴定  

Detection and Identification of Phytoplasma Strain Associated with Periwinkle Yellows

作　　者：蔡红[1] 孔宝华[1] 陈海如[1] 

机构地区：[1]云南农业大学云南省植物病理重点实验室,昆明650201

出　　处：《微生物学报》2003年第1期116-119,共4页Acta Microbiologica Sinica

基　　金：云南省自然科学基金 ( 2 0 0 0C0 0 1 4Q);云南省省院省校科技合作基金 ( 98YQ0 0 9)资助项目

摘　　要：A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.

关 键 词：长春花 黄化植原体 检测 鉴定 核糖体蛋白基因 

分 类 号：S432.4[农业科学—植物病理学]