肝复健冲剂抑制二乙基亚硝胺诱导大鼠肝癌形成的机制  

Experimental Study on Mechanism of Ganfujian Granule Inhibiting Hepatocarcinogenesis Induced with Diethylnitrosamine in Rats

作　　者：钱妍[1] 金岩[2] 李媛[2] 李柏[1] 凌昌全[1] 

机构地区：[1]第二军医大学附属长海医院上海市中医肝癌专病中心,上海200433 [2]第四军医大学附属口腔医院

出　　处：《浙江中医学院学报》2003年第2期56-58,共3页Journal of Zhejiang College of Traditional Chinese Medicine

摘　　要：目的 :探讨肝复健冲剂抑制大鼠肝癌发生发展的可能作用机制。方法 :应用肝复健冲剂干预二乙基亚硝胺(DEN)诱导大鼠肝癌过程 ,动态观察肝复健冲剂对肝癌诱发过程中肝细胞周期蛋白依赖性激酶(CDK4)、细胞周期素D1(cyclinD1)、c myc、IGF ⅡmRNA表达的影响。结果 :肝复健冲剂可延缓DEN诱导大鼠肝癌的发生、发展 ,降低实验大鼠肝癌发生率 ,延长其生存期 ;中药预防组大鼠肝细胞CDK4、cyclinD1阳性表达 ,c myc、IGF ⅡmRNA阳性信号均明显低于单纯诱癌组。结论 :肝复健冲剂可能通过抑制c myc、IGF Ⅱ的表达 ,影响细胞周期进程 ,阻断肝细胞失控增殖及癌变 。Objective:To study the mechanism of Ganfujian Granule inhibiting hepatocarcinogenesis in rats.Methods:Ganfujian Granule was adminisˉtered to interfere the hepatocarcinogenesis induced with diethylnitrosamine(DEN)in rats and the expression of cyclinˉdependentˉkinases4(CDK4),cyclinD1,cˉmyc and IGFˉⅡmRNA of hepatocyte was investigated during the period of hepatocarcinogenesis.Results:Ganfujian Granˉule can delay the process and reduce the rate of carcinogenesis,prolong rats'survival time.The expression of CDK4,cyclinD1,cˉmyc and IGFˉⅡmRNA of hepatocytes was much fewer in treatment group than that in control group.Conclusions:The mechanism of Ganfujian Granule inhibiting hepatocarcinogenesis may be relative to downˉregulate expression of cˉmyc and IGFˉⅡ,affecting cellˉcycl process and inhibiting the malignant proliferation and cancerization of hepatocytes.

关 键 词：肝复健冲剂 细胞增殖 细胞周期 原癌基因 中医药疗法 

分 类 号：R273.57[医药卫生—中西医结合]