脂质体介导胶质细胞源性神经营养因子基因转染对大鼠脊髓损伤后神经功能的影响  

Effect of liposome-mediated GDNF gene transfer in vivo on recovery of motor function following spinal cord injury in rats

作　　者：鲁凯伍[1] 陈哲宇[2] 侯铁胜[3] 傅强[3] 李明[3] 曹莉[2] 金大地[1] 

机构地区：[1]第一军医大学南方医院脊柱骨病科,广州510515 [2]第二军医大学基础医学部神经生物教研室 [3]第二军医大学长海医院骨科

出　　处：《上海医学》2003年第1期48-50,共3页Shanghai Medical Journal

基　　金：国家自然科学基金 (3 0 0 0 0 0 48);国家重点基础研究"973"基金 (19990 5 40 0 5 );全军"十五"青年基金课题 (0 1Q0 80 )

摘　　要：目的　探讨阳离子脂质体介导的胶质细胞源性神经营养因子 (GDNF)基因体内转染对大鼠脊髓损伤后后肢运动功能恢复的影响。方法　雄性成年SD大鼠 30只 ,随机均分为绿色荧光蛋白 (GFP)对照组及GDNF治疗组。采用改良Nystr m法经后路压迫大鼠胸段脊髓模型 ,以直接注射法将脂质体DC Chol和重组质粒 pEGFP GDNFcDNA混合后注入大鼠损伤脊髓。HE染色观察伤后 4周伤段脊髓残存组织的面积 ,采用斜板试验和运动功能评分观察大鼠后肢运动功能的恢复情况。结果　大鼠脊髓损伤后 1～ 4周 ,GDNF治疗组后肢运动功能评分明显高于GFP对照组 ,两组间差异有显著性 (P <0 .0 5 )。伤后 4周 ,GDNF组伤段残存脊髓组织的面积大于对照组 (P <0 .0 1)。结论　脂质体介导GDNF基因体内转染能明显减少脊髓损伤后伤区的坏死和萎缩 。Objective To investigate the effect of liposome mediated glial cell line derived neurotrophic factor (GDNF) gene transfer in vivo on the recovery of locomotor function after spinal cord injury (SCI) in adult rats. Methods The SCI model of acute posterior compression of spinal cord was established according to the method of Nystrm and then the DC Chol liposomes and recombinant plasmid pEGFP GDNF cDNA complexes were injected into the injured site. The surface area of the remnant injured spinal cord tissue was detected by HE staining. The locomotor function of hindlimbs of rats was evaluated by inclined plane test and BBB locomotor scale. Results The locomotor function scales of rats in GDNF group were higher than these in GFP group within 1 to 4 weeks after SCI ( P <0.05). Four weeks after SCI, the surface area of the remnant injured spinal cord tissue in GDNF group was larger than that in SAL group ( P <0.01). Conclusion The results indicate that GDNF cDNA mediated by liposomes can enhance the restoration of hindlimb locomotor function and decrease the necrosis and atrophy of the injured spinal cord.

关 键 词：胶质细胞源性神经营养因子 脊髓损伤 功能 脂质体 基因治疗 

分 类 号：R651.2[医药卫生—外科学]