30只鸭子中甲型流感病毒的检测  

Detection of type A influenza virus among 30 cases of duck samples

作　　者：陆嘉良[1] 周晓明[1] 林玉尊[1] 居丽雯[1] 姜庆五[1] 顾惠心[1] 俞顺章[1] 潘浩 朱凤才 

机构地区：[1]复旦大学公共卫生学院流行病学教研室,上海200032 [2]江苏省卫生防疫站急性传染病科

出　　处：《中国公共卫生》2003年第3期271-272,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金 (30 2 0 0 2 33);复旦大学青年科学基金资助 ;上海市卫生局科学发展基金课题

摘　　要：目的　了解近期禽宰场流感病毒在鸭子中的携带情况。方法　在禽宰场随机选择正常健康状况待宰鸭子 3 0只 ,分别取咽和肛门混合拭子 ,使用鸡胚培养扩增 ,血凝法初筛 ,甲型流感病毒核蛋白 (NP)蛋白保守核酸序列作扩增鉴定。结果　在 3 0份样品中 ,经鸡胚培养扩增 ,1∶5 0的血凝法初筛 5 0 %为阳性 ,经核酸聚合酶链反应扩增鉴定10份样品中 ,9份为甲型流感病毒。结论　用本方法在正常鸭子中甲型流感病毒的可检出携带率约为 45 %。Objective To study the carrying rate of type A influenza among health ducks recently in one of Shanghai poultry sacrifice ground.Methods The cotton swab samples from mouth and anal of ducks were mixed to be cultured in egg embryo and to the potential influenza virus was amplified by using chicken blood agglutination.Then PCR amplification of conserved type A influenza virus NP protein nucleotide sequene was used to determinate type A virus infection.Results 50% of 30 samples were positive in 1∶50 blood agglutination test,9 of 10 samples amplified correctly by PCR were with type A influenza virus.Conclusion Approximate 45% of normal ducks could be detected with type A influenza virus by using the method.

关 键 词：检测 甲型流感病毒 PCR反应 鸭子 血凝法 

分 类 号：R373.13[医药卫生—病原生物学] R446.5[医药卫生—基础医学]