青霉素产生菌的原生质体融合  

Protoplasts fusion of penicillin producing strains

作　　者：翁艳军[1] 赵作棋[1] 徐建国[1] 王丽巍[1] 唐丽华[1] 

机构地区：[1]哈药集团制药总厂,哈尔滨150086

出　　处：《中国抗生素杂志》2003年第3期129-130,182,共3页Chinese Journal of Antibiotics

摘　　要：将细菌血红蛋白基因引入青霉素产生菌产黄青霉H 10 6中而获得了产黄青霉基因工程菌 1M 5。产黄青霉 2 0 # 是一个青霉素产量高但产孢子能力偏低的生产菌。为提高产黄青霉的产量及产孢子能力 ,降低产黄青霉对氧的需求 ,我们采用原生质体融合技术 ,将通过UV诱变处理后检出的产黄青霉基因工程菌 1M 5Met-缺陷型菌株和产黄青霉 2 0 # Arg-缺陷型菌株分别用Novozyme 2 3 4和CellulaseR 10 (1∶1)混合酶处理所得原生质体按 1∶1混合后加入 3 0 %聚乙二醇 60 0 0融合 ,产物分离纯化 ,得到融合株C 3 96# 菌株 ,其青霉素产量和产孢子能力都有所提高 ,对氧的需求有所降低。By introducing bacterial hemoglobin genes into penicillin producer Penicillium chrgsogenum H 106, Penicillium genes chrysogenum 1M5 was obtained. Penicillium chrysogenum 20 # is a production strain with high yield but sparse character. In order to increase the yield and spore forming of penicillin producing strain, and to decrease oxygen demand , the protoplasts fusion technology was performed. 1M5 Met - deficient strain of Penicillium genes chrysogenum which was gained through the mutagent of the ultra violet radiation (UV) and 20 # Arg - deficient strain of Penicillium chrysogenum were treated spearately with mixed enzyme (Novozyme 234∶Cellulase R 10=1∶1). The protoplasts were mixed at ratio of 1∶1, then 30% PEG6000 was added to fuse protoplasts. Fused strain C 396 # was produced. The result of shake flask test showed that the penicillin productivity and spore forming of strain C 396 # were increased, while oxygen demand was decreased.

关 键 词：产黄青霉 原生质体融合 青霉素 

分 类 号：TQ465.1[化学工程—制药化工]