黄毛草莓、东北草莓与凤梨草莓种间杂交种的胚拯救  

Embryo Rescue of Hybrid between Fragaria nilgerrensis Schidl.,Fragaria mandschurica Staudt.and Fragaria ananassa Duch.

作　　者：马鸿翔[1] 陈佩度[1] 余桂红[2] 

机构地区：[1]南京农业大学农业部作物细胞遗传重点实验室,江苏南京210095 [2]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《江苏农业学报》2003年第1期37-40,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK2001417)

摘　　要：　以中国原产的2个草莓野生种黄毛草莓(FragarianilgerrensisSchidl.)和东北草莓(FragariamandschuricaStaudt)分别与凤梨草莓(FragariaananassaDuch.)杂交,对杂交后的幼胚进行胚拯救。研究了胚发育天数、培养基等对幼胚离体培养成苗的影响,明确了胚拯救条件:授粉后20～25d,MS培养基加入1mg/LBA、5mg/L2,4 D和0 5g/L水解酪蛋白。Two wild strawberry species Fragaria nilgerrensis and Fragaria mandschurica originated from China were crossed with Fragaria ananassa.Embryo rescue of interspecific hybrid was studied,and hybrids were obtained.Rate of plantlet was effected by culture medium,period of embryo development and different parental combination.The frequency of plantlet was the highest on MS medium with addition of 1 mg/L BA,5 mg/L 2,4D and 05 g/L casein hydrolysate.Optimum time was from 21 d to 25 d after pollination.

关 键 词：凤梨草莓 黄毛草莓 东北草莓 种间杂交种 胚拯救 培养基 亲本组合 

分 类 号：S668.4[农业科学—果树学]