ATB-FUNGUS 3酵母样菌抗真菌药物敏感性试验与ROSCO纸片扩散法及标准的CLSI M27-A2方法比较  

In vitro susceptibilities to yeasts using the ATB FUNGUS 3 method,compared with ROSCO disk diffusion testing and standard CLSI (NCCLS) M27-A2 methods

作　　者：吕火祥[1] 周永列[1] 朱永泽[1] 胡庆丰[1] 沈蓓琼[1] 张玉霞[1] 

机构地区：[1]浙江省人民医院检验医学中心

出　　处：《浙江检验医学》2009年第2期3-5,共3页Zhejiang Journal of Laboratory Medicine

基　　金：浙江省科技厅攻关计划项目2006C33014;浙江省医学重点学科建设基金07-010

摘　　要：目的探讨商品化的真菌药物敏感性检测ATB-FUNGUS3(ATB-F3法)及ROSCO纸片扩散法(Rosco法)与标准的CLSIM27-A2(标准MIC法)方法的符合率。方法分别用法国生物梅里埃的ATB-F3法和丹麦Rosco法及标准MIC法平行检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物对184株临床分离的念珠菌的体外药物敏感性,并进行比较。结果ATB-F3法与标准MIC法检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物的符合率分别为100%、100%、96.2%、92.9%、96.7%;Rosco法与标准MIC法的符合率分别为85.9%、91.8%、94.6%、89.7%、90.2%;ATB-F3法与ROSC法的符合率分别85.9%、91.8%、94%、87.5%、88%。结论ATB-F3法及Rosco法与MIC法均有很高的符合率,而以ATB-F3法更高;两种商品化的方法间符合率较好,临床实验室均可选用,但宜选用MIC法或ATB-F3法以尽可能减少误差。Objective To compare the concordance rate between the microbroth ATB-FUNGUS 3 (ATB-F3) method and ROSCO disk diffusion testing for in vitro determination of the susceptibility of Candida spp.,taking CLSI M27-A2 as the reference method.Methods ATB-FUNGUS 3 method and Rosco disk diffusion testing and CLSI M27-A2 method were applied parallel in detecting the susceptibility of 184 strains of Candida to 5 kind of antifungal agents and the results were compared.Results The concordance rates between ATB-FUNGUS 3 method and CLSI M27-A2 method for antifungal susceptibility testing about 5-flucytosine,amphotericin B,fluconazole,itraconazole and voriconazole were 100%,100%,96.2%,92.9%,96.7%,respectively. Between Rosco disk diffusion testing and CLSI M27-A2 method,the concordance rates were 85.9%,91.8%,94.6%,89.7%,90.2%,respectively.Between ATB-FUNGUS 3 method and Rosco disk diffusion testing,the concordance rates were 85.9%,91.8%,94.0%,87.5%,88.0%,respectively.Conclusion The concordance was good between ATB-FUNGUS 3 (ATB-F3) method and ROSCO disk diffusion testing and CLSI M27-A2 method,but ATB-FUNGUS 3 was better than the ROSCO method.We recommend ATB-FUNGUS 3 method for clinical laboratories to avoid the error.

关 键 词：抗真菌药物 药物敏感试验 MIC法 纸片扩散法 

分 类 号：R446.5[医药卫生—诊断学]