人肠上皮细胞内毒素低反应性及其机制探讨  

LOW RESPONSIVENESS OF CULTURED HUMAN INTESTINAL EPITHELIAL CELLS TO LPS STIMULATION AND ITS POSSIBLE MECHANISM

作　　者：张道杰[1] 蒋建新[1] 陈永华[1] 段朝霞[1] 熊健琼[1] 朱佩芳[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,重庆400042

出　　处：《解放军医学杂志》2003年第3期194-196,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展规划项目资助课题 (编号G1 9990 540 3)

摘　　要：为探讨人正常肠上皮细胞对内毒素(LPS)刺激低反应性的机制 ,用ELISA法检测LPS刺激肠上皮细胞 18h后IL 8的分泌水平 ,用RT PCR及RPA法检测肠上皮细胞LPS跨膜信号转导相关受体TLR4、TLR2、CD14和MD2mRNA的表达及LPS刺激对其表达的影响。结果表明 ,LPS刺激不能引起肠上皮细胞IL 8的分泌 ,而IL 1β刺激却能引起其分泌 ;人正常肠上皮细胞TLR4、TLR2、CD14和MD2mRNA的表达均较弱。LPS刺激后 ,TLR4、CD14和MD2mRNA表达进一步降低 ,而TLR2的表达无显著变化。说明肠上皮细胞对LPS的低反应性与细胞表面LPS相关受体低表达有一定的内在联系 ,从而进一步证实TLR4、CD14。To investigate the mechanism of low responsiveness of cultured human intestinal epithelial cells (IECs) to LPS stimulation, IL-8 secretion level was detected by ELISA in supernatants harvested after the IECs were treated with LPS for 18h, and TLR4, TLR2, CD14 and MD2 mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR) and RNase protection assay(RPA). It was found that IL-8 was not detectable after IECs were treated with LPS and the expression levels of TLR4, TLR2, CD14 and MD2 mRNA of IECs were low and after exposed to LPS, the expressions of TLR4, CD14 and MD2 mRNA of IECs became further lower, whereas the expression of TLR2 mRNA didn't change obviously. The result suggests that low expressions of LPS signal transduction-associated receptors on IECs may be responsible for low response of IECs to LPS, so it confirms further that TLR4, CD14 and MD2 play important roles in transmembrane LPS signal transduction.

关 键 词：肠上皮细胞 内毒素信号转导相关受体 逆转录聚合酶链反应 核糖核酸酶保护法 

分 类 号：R378[医药卫生—病原生物学]