重组腺病毒和质粒对心肌缺血治疗效果的比较  

The comparative efficacy of recombinant adenovirus and plasmid in treatment of myocardial ischemia

作　　者：张友荣[1] 吴丹莉[1] 劳妙芬[1] 毕建进[1] 吴祖泽[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2003年第1期16-18,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家"8 63"计划资助项目 ( 2 0 0 1AA2 170 61)

摘　　要：目的 :研究不同载体对基因治疗效果的影响。方法 :采用本实验室建立的犬心肌缺血动物模型 ,通过冠状动脉造影和TTC染色两种方法比较了携带肝细胞生长因子基因的重组腺病毒Ad_HGF和质粒puDKH对心肌缺血的治疗效果 ;并且在体外采用绿色荧光蛋白报告基因测定了不同载体对原代心肌细胞的转染率。结果 :重组腺病毒Ad_HGF对心肌细胞的转染率和心肌缺血的治疗效果均好于重组质粒puDKH。结论Objective:To compare the therapeutic effects of recombinant adenovirus and plasmid on myocardial ischemia.Methods:In canine myocardial ischemia model,the therapeutic effects of recombinant adenovirus Ad_HGF and plasmid puDKH were compared using coronary artery angiography and TTC staining. Moreover, the transfer efficiency of recombinant adenovirus and plasmid was examined by GFP(green fluorescence protein)report gene.Results:The therapeutic efficacy of Ad_HGF was better than puDKH and the transfer efficiency of Ad_HGF in primary myocardium was higher than plasmid.Conclusions:Gene transfer system is one of the key factors influencing the efficacy of gene therapy.

关 键 词：腺病毒科 重组质粒 基因疗法 心肌缺血 治疗结果 

分 类 号：R541.4[医药卫生—心血管疾病]