人源性角质细胞生长因子(KGF)的克隆及其表达分析  

Cloning of human keratinocyte growth factor(KGF) and analysis of its expression in E.coli

作　　者：傅成波[1] 赵蓓蕾[1] 王宝勤[1] 罗庆良[1] 熊国林[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2003年第1期22-24,29,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的 :克隆出正确的人源性角质细胞生长因子 (KGF)并探讨影响其表达的因素。方法 :应用RT_PCR技术克隆出KGF基因 ,并用pBV2 2 0表达质粒对其进行表达 ,然后用RNAdraw对其RNA进行分析。结果 :带有信号肽的KGF基因在pBV2 2 0中表达量为 10 %左右 ,而去掉信号肽的KGF基因在pBV2 2 0中表达量估计只有 1%～ 2 % ;利用RNAdraw分析的结果表明前者RNA前端没有形成茎环结构 ,而后者则形成了茎环结构。结论 :KGF基因在细菌中表达量较低 。Objective:To clone the correct KGF gene and study the factor influencing its expression.Methods:KGF gene was cloned by RT_PCR, and expressed in pBV220.The KGF RNA was analysed by RNAdraw software.Results:The expression of KGF with its signal sequence was about 10%, but 1%-2% without its signal sequence.RNAdraw software showed that the beginning of the RNA of the latter formed the stem_ring structure,while that of the former did not.Conclusions:The expression of KGF gene is very low in E.coli and its signal sequence is a very important factor for its expression.

关 键 词：角蛋白细胞 角质细胞生长因子 基因表达 信号肽类 

分 类 号：R346[医药卫生—基础医学]