部分结构特殊多肽的纳升电喷雾串联质谱测序分析  

Some special peptides sequence analysis by nano-ESI-MS/MS

作　　者：王红霞[1] 何昆[1] 李卫华[1] 魏开华[1] 王杰[1] 杨松成[1] 张学敏[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《军事医学科学院院刊》2003年第1期36-39,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 3 77)

摘　　要：目的 :测定用Edman降解方法不能测序的结构特殊多肽的序列。方法 :用纳升电喷雾四极杆_飞行时间串联质谱的denovo测序方法进行测序。结果 :测定了亮丙瑞林、胸腺五肽及EPD等的多肽序列 ,这些多肽用Edman降解方法未能测定出序列。结论 :串联质谱测序法是Edman降解测序法的最好补充 ,且具有灵敏、快速和样品用量少的优点。Objective:To analyze the sequence of some special peptides which can not be sequenced by Edman degradation method.Methods and Results:Peptides were sequenced by de novo sequencing method of nano_ESI_MS/MS.Leuprorelin, thymopoietin and EPD were sequenced by this method, which had not been sequenced by Edman degradation method because of their special amino acid modifications or compositions.Conclusion:Nano_ESI_Q_TOF_MS/MS is the best complementary method to Edman degradation method in peptide sequencing because this method has no restriction to N_terminal modification and has high sensitivity, high mass accuracy and high speed.

关 键 词：纳升电喷雾四极杆-飞行时间串联质谱 氨基酸序列 

分 类 号：Q516[生物学—生物化学]