锯缘青蟹胸神经团的免疫细胞化学定位研究  

IMMUNOCYTOCHEMICAL LOCALIZATION IN THE THORACIC GANGLION MASS OF THE MUD CRAB, Scylla serrata

作　　者：黄辉洋[1] 叶海辉[2] 李少菁[1] 王桂忠[1] 金朱兴[1] 

机构地区：[1]厦门大学海洋系,厦门361005 [2]厦门大学生命科学学院细胞生物学研究室,厦门361005

出　　处：《海洋科学》2003年第3期77-80,共4页Marine Sciences

基　　金：福建省重中之重项目"福建省海洋生物优良种质和生物活性物质的应用基础研究";高等学校博士学科点专项科研基金项目20010384010号

摘　　要：采用兔抗哺乳类抗血清 ,应用链霉菌抗生物素蛋白 -过氧化物酶免疫细胞化学技术 ,研究了锯缘青蟹(Scyllaserrata)胸神经团中5 羟色胺 (5 HT)、胰高血糖素(Glu)和神经肽Y(NPY)的分布。其分布的主要特征为 :5 HT免疫阳性细胞在每对步足神经节都呈对称排列 ;Glu免疫阳性最强 ,胸神经节的阳性细胞排列成两条环绕胸动脉孔分布的细胞带 ,神经髓质中免疫阳性物质呈斑块状分布 ;NPY阳性细胞主要分布于食道下神经节。Immunocytochemistry techniques were applied to observing the immunoreactive neurons and neuropils of serotonin (5 HT), glucagon (Glu) and neuropeptide Y (NPY) in the thoracic ganglion mass of Scylla serrata. The distribution characteristics of the 3 immunoreactive substances are as follows: 5 HT immunoreactive cells in each leg ganglion are symmetrically distributed; Glu immunoreactivity is strong;the immunoreactive cells in the thoracic ganglionare arrange in two distinct bands, which surround the thoracic artery foramen; Glu immunoreactivity in the neuropils is distributed in patchness;most of NPY immunoreactive cells locate in the sub esophageal ganglion. The specific distribution patterns of the 3 immunoreactive substances may provide morphological proofs for their different neurophysiological function and the evolution of the abdomen neural chain in decapod.

关 键 词：锯缘青蟹(Scylla serrata) 胸神经团 5-羟色胺 胰高血糖素 神经肽Y 免疫细胞化学 

分 类 号：Q189[生物学—神经生物学]