红莲优6号杂交水稻与亲本三系不同发育时期幼苗叶片基因表达差异分析  

Differential Display of Gene Expression in Hybrid Rice Honglianyou-6 and Its Parental Three-line Seedling Leaves in Different Developmental Stages

作　　者：张义平[1] 陈学峰[1] 熊建华[1] 朱英国[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2003年第1期27-30,共4页Journal of Wuhan Botanical Research

基　　金：国家 973资助项目 ( 2 0 0 1CB10 880 6 )

摘　　要：运用 DDRT- PCR对红莲型杂交稻组合红莲优 6号及其亲本、保持系 (粤泰 A、粤泰 B、9311)的一叶期、三叶期叶片基因表达状况进行分析。结果表明 :杂种与亲本之间在同一发育时期基因表达既有质量上又有数量上的差异 ,但差异表达基因所占比例较小 ;而对于同一材料 ,一叶期、三叶期的叶片 c DNA扩增带型相似 ,没有发现基因质上的差异表达 ,说明一叶期与三叶期幼苗叶片基因表达差异很小。实验也证明 DDRT- PCR技术结合银染方法是一种简单快速分析差异表达基因的有效方法。The gene expression in leaves for hybrid rice,Honglianyou-6,and its parental lines(YTA,YTB,9311) at different development stages was analyzed by using DDRT-PCR.Our results revealed that both quality and quantity difference in gene expression were detected among hybrid and parental lines at same developmental stage.Although gene expression in hybrid is obviously altered,the differential expressed gene still occupy a very small proportion of total expressed gene;In addition,no qualitative difference in gene expression was observed between one-leaf stage and tri-leaf stage in the same material.This indicate the great similarity of gene expression in the two stages.Also,our experiment further provide evidence that DDRT-PCR technique with silver staining is a rapid,and convenient method for detecting differentially expressed genes.

关 键 词：红莲优6号 杂种优势 基因表达 幼苗 mRNA差异展示 

分 类 号：Q943[生物学—植物学]