长春花黄化植原体核糖体蛋白基因片段序列分析  

SEQUENCS ANALYSIS OF RIBOSOMAL PROTEIN GENE OF PHYTOPLASMA ASSOCIATED WITH PERIWINCLE YELLOWS

作　　者：蔡红[1] 陈惠[1] 李凡[1] 陈海如[1] 

机构地区：[1]云南农业大学云南省植物病理重点实验室,昆明650201

出　　处：《微生物学通报》2003年第1期34-37,共4页Microbiology China

基　　金：云南省自然科学基金 (No . 2 0 0 0C0 0 1 4Q);云南省省院省校科技合作基金资助项目 (No . 98YQ0 0 9)

摘　　要：对自然表现典型黄化症的长春花植株总DNA进行植原体核糖体蛋白基因 (ribosomalproteingene ,rpgene)PCR扩增 ,得到约 1 3kb的特异片段。将此特异片段与pGEM TEasy载体连接并转化到大肠杆菌JM 1 0 9感受态细胞中 ,通过PCR鉴定、限制性内切酶 (EcoRI)酶切分析、核苷酸序列测定及分析 ,结果表明该株系核糖体蛋白基因片段长 1 ,2 4 4bp ,包含rpl2 2、rps3基因 ,分别编码 1 2 9和 2 5 2个氨基酸 ,且这两个基因为重叠基因。A 1 3kb DNA fragment was amplified in DNA samples extracted from periwinkle showed yellows The amplified fragment was ligated into pGEM T easy vector and then transformed into JM109 strain of E coli Cloned DNA fragments were verified by PCR, restriction endonuclease ( Eco RI) digestion and sequence analysis The result revealed that the ribosomal protein gene of PY strain consists of 1244 base pairs, which contains the entire rp l22 gene encoding 129 amino acids and rps3 gene encoding 252 amino acids These two genes are overlap The character of ribosomal protein gene of PY strain is very resemble with that of other phytoplasmas

关 键 词：长春花 黄化 植原体 核糖体蛋白基因 序列分析 

分 类 号：S432[农业科学—植物病理学]