家蚕质多角体病毒片段IV的全序列测定  

SEQUENCING OF WHOLE SEGMENT IV OF BOMBYX MORI CYTOPLASMIC POLYHEDROSIS VIRUS

作　　者：林伟[1] 杨文利[2] 徐安龙[2] 梁玉尧[1] 陈森雄[1] 戴伟君[1] 张景强[1] 

机构地区：[1]中山大学生物防治国家重点实验室,广州510275 [2]中山大学海洋生物功能基因组开放实验室,广州510275

出　　处：《微生物学通报》2003年第1期41-44,共4页Microbiology China

基　　金：国家自然科学基金结构生物学倾斜项目 (No 3 0 0 70 1 69)

摘　　要：在随机引物建库的基础上 ,通过交叉设计引物及加单链DNA接头 ,应用RT PCR技术克隆了家蚕质多角体病毒dsRNA片段Ⅳ的全长cDNA ,并测定了它的全序列。该片段长 3,2 6 2bp ,含有一个完整的开放读码框 ,编码一个长 1 ,0 5 8氨基酸的成熟多肽。序列分析表明 ,该片段与日本BmCPV片段Ⅳ的核苷酸序列同源性为 89% ,氨基酸序列同源性为 95 %。Based on the preparation of cDNA library using random oligonucleotide, a series of primers overlapping the whole segment Ⅳ of BmCPV and an adaptor of ssDNA were designed BmCPV dsRNA segment Ⅳ was acquired by RT PCR amplification and sequenced Segment Ⅳ was 3,262 bp long and had a single,long open reading frame BmCPV segment Ⅳ encoded a polypeptide with 1,058 amino acids Sequences analyses showed that it was homologous 89% and 95% to BmCPV dsRNA segment Ⅳ of Japan strains in nucleotides and amino acids,respectively

关 键 词：家蚕质多角体病毒 BmCPV 双链RNA RT-PCR 序列测定 

分 类 号：S884[农业科学—特种经济动物饲养]