球形芽孢杆菌Mtx1杀蚊毒素在苏云金芽孢杆菌以色列亚种中的表达  

EXPRESSION OF MOSQUITOCIDAL MTX1 TOXIN GENE FROM BACILLUS SPHAERICUS IN CRYSTAL-MINUS B.THURINGIENSIS SUBSP. ISRAELENSIS

作　　者：张蓓华[1] 刘海洲[1] 刘铭[1] 闫建平[1] 袁志明[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071

出　　处：《微生物学通报》2003年第1期49-52,共4页Microbiology China

基　　金：国家自然科学基金资助项目 (No 39770 1 70 );中国科学院生物技术特别支持项目 (No STZ 0 0 2 7)~~

摘　　要：将来源于球形芽孢杆菌SSII 1的mtx1毒素基因克隆至穿梭载体 pBU4上 ,得到mtx1插入方向相反的重组质粒 pMT9和pMT4。含有 pMT9和 pMT4的大肠杆菌转化子能表达产生Mtx1毒素 ,发酵液对敏感和抗性致倦库蚊幼虫具有中度毒杀作用 ;含有pMT9和pMT4的苏云金芽孢杆菌转化子B pMT9和B pMT4在营养体生长阶段对敏感蚊幼和抗性幼虫也具有毒性 ,毒力与野生型SSII 1相当 ,而不同转化子在芽孢形成期的毒力因插入的mtx1基因转录方向不同而表现出差异 ,其中B pMT4对目标蚊幼毒力极低 (LC50 >1 0 0mg/mL) ,而B pMT9对蚊幼虫具有毒性 (LC50 =2 4 9mg/mL)。The mtx1 gene originated from Bacillus sphearious SSII 1 was cloned to shutle vector pBU4 Two recombinant plasmids pMT9 and B pMT4 were obtained, with the inserted fragments in the opposite orientations The two E coli transformants have the same toxicity against both the resistant and susceptible Culex quinquefasciatus larvae Two B thuringiensis subsp isralensis recombinants, which contain recombinants plasmid pMT4 and pMT9 respectively,were constructed by electroporation The bioassay results showed that the recombinants B pMT9 and B pMT4 have toxicity to resistant and susceptible mosquito larvae during vegetative growth stage,having the LC 50 values similar to that of SSII 1 However,the toxic levels of the final sporulated cultures of recombinants B pMT4 and B pMT9 differ,with a LC 50 value of 2 49mg/mL for B pBT9 and no toxicity for B pMT4

关 键 词：球形芽孢杆菌SSII-I Mtx1毒素 苏云金芽孢杆菌以色列亚种 杀蚊毒素 

分 类 号：Q786[生物学—分子生物学]