南四湖泥鳅和大鳞副泥鳅随机扩增多态DNA分析初报  

RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS(RAPD) OF MISGURNUS ANGUILLICAUDATUAND PARAMISGURNUS DABRYANUS FROM NANSI LAKE

作　　者：李殿香[1] 王金星[2] 王来元[3] 

机构地区：[1]山东济宁师专生物系,济宁272125 [2]山东大学生命科学院 [3]中国协和医科大学基础医学研究所

出　　处：《四川动物》2003年第1期6-9,共4页Sichuan Journal of Zoology

摘　　要：采用随机扩增多态性DNA(RAPD)技术对南四湖的泥鳅 (Misgurnusanguillicaudatus)和大鳞副泥鳅(Paramisgurnusdabryanus)进行了初步分析。共用 10个 10bp的OPG组寡核苷酸片段作为引物 ,筛选出结果好的 5个引物 ,从泥鳅 4个体基因组DNA中扩增出 142个片段 ,共有片段 12个 ,特有片段 5个 ;从大鳞副泥鳅 3个体基因组DNA中扩增出 10 1个片段 ,共有片段 18个 ,特有片段 5个 ,在总扩增出的 2 43个片段中 ,共有片段仅 4个。所有扩增片段大小介于 0 2～ 3kb之间。用PoPGen32软件计算出个体间的遗传距离 ,依此进行UPGMA聚类得到的分子系统树与形态分类学的结果一致 ,这 7个体被聚成 2大类 ,正对应着形态学的 2个种 :泥鳅和大鳞副泥鳅。The genetic differentiation of Misgurnus anguillicaudatus and Paramisgurnus dabryanus from Nansi Lake,Shandong Province was studied by RAPD.Totally 243 amplified DNA fragments (200～3000bp) were obtained by using 5 random oligonucleotide primers from 7 samples of the two kinds of fish.Of them 4 fragments were shared by the 7 samples,18 fragments by 3 samples of Paramisgurnus dabryanus and 12 fragments by 4 samples of Misgurnus anguillicaudatus. RAPD phenogeam was constructed by UPGMA using genetic distance of samples calculated by PoPGen32 software.It has been suggested that the 7 samples were divided into 2 clusters,which is consistent with the results based on morphological study in which they belong to Misgurnus anguillicaudatus and Paramisgurnus dabryanus.

关 键 词：泥鳅 大鳞副泥鳅 RAPD分析 

分 类 号：Q953[生物学—动物学]